Terminal progress report, biological effects of nitrous oxide as used in dentistry.
American Dental Assn Health Fdn, Chicago, Illinois, 1981 Jan; :1-60
Teratogenic and mutagenic effects of nitrous-oxide (10024972) (N2O) were studied in rats and mice. Sprague-Dawley-rats were exposed continuously or intermittently to up to 20 percent N2O 5 days per week on days 10 through 15 or 1 through 21 of gestation. Animals were observed for clinical symptoms and examined for pathological changes at the end of the study period. Offspring of pregnant dams were examined for evidence of genotoxic changes. Male AJ-mice and male Sprague-Dawley-rats were exposed to 5 or 20 percent N2O for up to 3 months and were mated to unexposed females at selected intervals. Offspring were assayed for genotoxic effects. Pregnant rats exposed to 5 to 20 percent N2O showed dose related decreases in maternal body weights, increases in resorptions, decreases in litter size and fetal weights, and increases in skeletal abnormalities. N2O at 5 percent was a no effect dose. Adverse effects did not occur with intermittent N2O exposure or when the exposure was limited to days 10 through 15 of gestation. Exposure of mice to 5 or 20 percent N2O caused dose related decreases in litter size and increases in the number of resorptions. No significant genotoxic effects occurred in the rats.
NIOSH-Grant; Animal-studies; Anesthetics; Dentistry; Employee-exposure; Reproductive-hazards; Toxicopathology; Biological-effects; Health-hazards
Final Grant Report
NTIS Accession No.
American Dental Assn Health Fdn, Chicago Illinois
Research Institute American Dental Assoc Hlth FDn211 East Chicago Avenue Chicago, IL 60611