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Analysis of organic pesticides by gas chromatography.

Public Health Service, U.S. Department of Health, Education and Welfare, Research Report No. 63/12-155, NTIS PB-227-567 1963 Nov; :1-60
Two chromatographic and one enzymatic procedures were investigated for screening organic pesticides. Chlorinated organic pesticides dissolved in petroleum-ether were spotted on a standard chromatographic paper and dried. The spots were developed in a saturated atmosphere of a mobile solvent for 1.5 hours and visualized following treatment with silver-nitrate under ultraviolet light. In another procedure, organophosphates were treated similarly except the compounds were dissolved in ethyl-acetate or petroleum-ether, developed in a bromine saturated atmosphere for 30 seconds, and visualized by spraying with dilute fluorescein under ultraviolet light. For enzymatic quantitation, the compound was dissolved in benzene, mixed with glycerol-methanol solutions, and acetylcholinesterase was added, followed 60 minutes later with acetyl-bromide. Gas chromatography was also used to analyze several compounds at various concentrations. The number of peaks and retention time at various instrumental settings were examined. For the chlorinated insecticides and herbicides analyzed by the first chromatographic method, the limit of detection was 2 micrograms. Limitations of the method included identical values obtained for many compounds and insensitivity of a few. Organic pesticides analyzed by the second method were of no qualitative value. Its limit of detection was 5 micrograms. For the pesticides assayed enzymatically, the quantity of two or more compounds present could not be determined accurately. The detection sensitivity by gas chromatographic analysis was equal to or greater than paper chromatographic methods. The electron capture detection method was 200 times more sensitive than the hydrogen flame detector. The authors conclude that electron capture gas chromatography can analyze pesticides reliably.
NIOSH-Grant; Industrial-chemicals; Analytical-chemistry; Poisons; Gas-chromatography; Safety-research; Occupational-health; Pesticides; Fumes
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Public Health Service, U.S. Department of Health, Education and Welfare, Research Report No. 63/12-155, NTIS PB-227-567