Machine Oils And Nitrosamines Study For Carcinogenesis In Mice.
Mulligan-LT; Williams-S; Plankenhorn-LJ; Garner-FM
NIOSH 1982 Sep:151 pages
The effects of 11 synthetic machine cutting oils were examined in mice. Each oil was analyzed for diethanol-N-nitrosamine (1116547) by gas chromatography. Groups of Agouti-C3H/HEJ-mice received 0.05 milliliters of each oil topically twice weekly for 98 weeks. Each group consisted of 50 males and 50 females. During the treatment period, mice were observed for clinical symptoms. Gross and microscopic pathological studies were conducted at the end of the experiment. A high incidence of deaths were seen in mice given cutting oil number 4 which contained the highest measured concentration of diethanol-N-nitrosamine at 4.57 milligrams per gram. Treatment site masses were observed in all groups except controls. Nontreatment site tumors was seen in all females, located primarily in the mammary glands. Hepatocellular neoplasia was the only significant histological finding; it was seen most frequently in mice given cutting oil number 4 and cutting oil number 6. The incidence of proliferative liver lesions was higher in females than in males. The authors conclude that the materials in cutting oils 4 and 6 may be oncogenic to the liver. The authors also conclude that screening programs are necessary to measure methemoglobin concentrations in workers handling these oils.
NIOSH-Contract; Machine-shop-workers; Animal-studies; Hepatotoxicity; Toxic-effects; Occupational-exposure; Employee-exposure; Pathogenicity; Medical-research; Contract-210-77-0136;
NTIS Accession No.
Division of Biomedical and Behavioral Sciences, NIOSH