Disodium tetrachloropalladate (Na2PdCl4), an inhibitor of rat liver mitochondrial electron transport.
Biagini-RE; Moorman-WJ; Winston-GW
Toxicol Lett 1982 Jul; 12(2-3):165-170
The interaction of disodium-tetrachloropalladate (13820536) (Na2PdCl4) with isolated electron transport and energy metabolism was studied in Sprague-Dawley-rats. Adenosine-triphosphatase (ATPase) activity was assayed by the determination of proton ejection following addition of carbonyl-cyanide-m- chlorophenylhydrazine (555602) (CCCP) or Na2PdCl4. Phosphate were performed after the addition of 400 nanomoles of adenosine- stimulate proton ejection. At the highest concentrations, 64 micromolar, state 3 respiration rates with both succinate and mixture of 5 millimolar (mM) glutamate, 1mM malate, 1mM malonate were inhibited about twenty and forty percent of control values, respectively. Na2PdCl4 demonstrated no ability to stimulate state 4 respiration of ATPase. Along with an inhibition of the electron transport chain, Na2PdCl4 suppressed the rate of accumulation of protons during ADP phosphorylation. The authors conclude that Na2PdCl4 is a relatively potent inhibitor of mitochondrial electron transport and there is a multiphasic inhibitor interaction with the rat liver mitrochondrial electron transport system which yields primary inhibitors of respiration without observable interaction with the coupling system mechanics.
NIOSH-Author; Analytical-methods; Analytical-chemistry; Bioassays; Laboratory-techniques; Chemical-analysis; Analytical-instruments