Hairless mice as models for chloracne: a study of cutaneous changes induced by topical application of established chloracnegens.
Puhvel-SM; Sakamoto-M; Ertl-DC; Reisner-RM
Toxicol Appl Pharmacol 1982 Jul; 64(3):492-503
Cutaneous changes induced by topical application of established chloracnegens were studied in female SKh:HR-1-hairless-mice and HRS/J-hairless-mice. Solutions of 0.1 milliliter of polychlorinated naphthalenes, polychlorinated biphenyls and 2,3,7,8- tetrachlorodibenzo-p-dioxin (1746016) were dissolved in acetone and applied to the dorsal skin of the mice for a treatment period ranging from 2 to 10 weeks. The cutaneous changes were monitored during this time with punch biopsies of treated skin taken for histologic studies at regular intervals. Mice were killed at the end of the treatment. Most chloracnegens caused a pattern of cutaneous pathology without associated hyperkeratinization of the sebaceous follicles. Only 3,4,3',4' tetrachlorobiphenyl induced a true chloracne like syndrome. This syndrome caused massive hyperkeratosis of the sebaceous follicles and hyperkeratinization of intradermal pilar cysts further compounded by gross weight gain.
NIOSH-Publication; NIOSH-Grant; Analytical-methods; Analytical-chemistry; Laboratory-techniques; Chemical-analysis; Histochemical-analysis; Biochemical-tests; Laboratory-animals
Medicine University of California 405 Hilgard Avenue Los Angeles, Calif 90024
Toxicology and Applied Pharmacology
University of California Los Angeles, Los Angeles, California