Tier II Mutagenic Screening of 13 NIOSH Priority Compounds, Individual Compound Report, 2-Nitropropane.
NIOSH 1981 Jul:193 pages
2-Nitropropane (79469) (2NP) was subjected to a tier II mutagenic test screening program. The screening assays included unscheduled DNA synthesis (UDS) assay in human diploid fibroblasts with exposures of 3 hours duration and concentrations up to 5000 micrograms per milliliter of culture medium; dominant lethal test in male rats with exposure to atmospheres containing 25 or 200 parts per million (ppm) 2NP for 7 hours per day for 5 days; sperm abnormality test in male mice using the same conditions; cytogenetic test in male and female rat bone marrow cells using the same exposure conditions; or a single exposure of 7 hours duration; and sex linked recessive lethal test in Drosophila-melanogaster with exposure to atmospheres of 700ppm for 4.5 hours. There was no increase in UDS in cells treated with 2NP nor were there increases in the frequencies of chromosomal aberrations in male or female rats. There were no large effects attributable to 2NP in the dominant lethal test on pregnancy frequency, number of corpora lutea or implantations, or the frequency of early deaths. Reductions to 75 percent were seen in pregnancy frequencies in weeks 1 and 5 of the 200ppm atmosphere group and in the frequencies of live implantations or live implantations and late deaths in week 2 of the 200ppm atmosphere group. The author concludes that there was no evidence of genotoxic activity.
NIOSH-Contract; Contract-210-78-0026; Nitro-compounds; Toxicology; Mutagenesis; Bioassays; Screening-methods; Chromosome-damage;
NTIS Accession No.
Inveresk Research International Limited, Musselburgh, Scotland, NIOSH, Cincinnati, Ohio, No. 31, 193 pages, 14 references