Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Involvement of the sodium-potassium pump in the mechanism of postjunctional supersensitivity of the vas deferens of the guinea pig.

Authors
Gerthoffer-WT; Fedan-JS; Westfall-DP; Goto-K; Fleming-WW
Source
J Pharmacol Exp Ther 1979 Jan; 210(1):27-36
NIOSHTIC No.
00118624
Abstract
The relationship between the electrogenic sodium-potassium (NaK) pump and postjunctional supersensitivity of guinea-pig vas deferens was studied. The vasa deferentia were removed from male guinea- pigs, desheathed, and exposed to ouabain (630604) followed by methoxamine (390283). Pairs of vasa deferentia were either unoperated or surgically denervated. The effects of ouabain or reduced extracellular potassium were determined. Nuclear, mitochondrial, microsomal, and supernatant fractions of homogenized vasa deferentia were assayed for NaK adenosinetriphosphatase (ATPase). Neither ouabain nor reduced potassium depolarized the cell membranes of denervated vasa deferentia, nor did ouabain enhance methoxamine sensitivity. Denervation of the vasa deferentia resulted in reduced ATPase in the subcellular fractions. The authors suggest that interruption of the excitatory innervation of the vas deferens partially inhibits the NaK pump by reducing ATPase activity. Inhibition of the pump may cause depolarization of the cell membrane due to the electrogenicity of the pump.
Keywords
NIOSH-Author; Laboratory-animals; Enzyme-activity; Cell-biology; Cellular-reactions; Reproductive-system
CODEN
JPETAB
CAS No.
630-60-4; 390-28-3
Publication Date
19790101
Document Type
Journal Article
Fiscal Year
1979
NTIS Accession No.
NTIS Price
Issue of Publication
1
Source Name
The Journal of Pharmacology and Experimental Therapeutics
TOP