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Genotoxic and mutagenic assays of halothane metabolites in bacillus subtilis and salmonella typhimurium.

Authors
Sachdev-K; Cohen-EN; Simmon-VF
Source
Anesthesiology 1980 Jul; 53(1):31-39
NIOSHTIC No.
00115380
Abstract
The genotoxic and mutagenic effects of halothane (151677) and its metabolites were studied. Standard mutagenicity assays using Salmonella-typhimurium were performed for halothane, 2-chloro-1,1- difluoroethylene (359104), and 2-bromo-1,1-difluoroethylene. Bacillus-subtilis strains H17 and M45 were grown and treated with test compounds. Toxicities and DNA interactions were assessed. Mutagenicity assays were also performed using polymerase deficient Escherichia-coli. Halothane and its metabolites were nonmutagenic in Salmonella, however, conjugates of these compounds were weakly mutagenic. In Bacillus-subtilis, halothane metabolites inhibited growth of the M45 strain which is deficient in DNA repair ability. There was no differential toxicity found in tests for DNA modifying activity in Escherichia-coli. The authors conclude that there is a possible relationship between halothane metabolism and reported toxic effects associated with occupational anesthetic exposures.
Keywords
NIOSH-Publication; NIOSH-Grant; Brominated-hydrocarbons; Anesthetics; Metabolites; Comparative-toxicology; Mutagenesis; Bioassays; Genetic-disorders; Metabolism
Contact
Anesthesia Stanford University 300 Pasteur Drive Stanford, Calif 94305
CODEN
ANESAV
CAS No.
151-67-7; 359-10-4
Publication Date
19800701
Document Type
Journal Article
Funding Amount
599304
Funding Type
Grant
Fiscal Year
1980
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00622
Issue of Publication
1
ISSN
0003-3022
Source Name
Anesthesiology
State
CA
Performing Organization
Stanford University, Stanford, California
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