Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Sister chromatid exchange in murine alveolar macrophages, regenerating liver and bone marrow cells - a simultaneous multicellular in vivo assay.

Authors
Conner-MK; Alarie-Y; Dombroske-RL
Source
Chromosoma 1979 Sep; 74(1):51-55
NIOSHTIC No.
00105214
Abstract
A method for simultaneously affecting sister chromatid exchange (SCE) in murine alveolar macrophages, regenerating liver and bone marrow cells of hepatectomized mice and in alveolar macrophages and bone marrow cells of nonhepatectomized mice was developed. Both nonhepatectomized and hepatectomized BDF1-mice were given 9 hourly, 2 milliliter injection of bromodeoxyuridine (59143) (BrdU). The following day, the animals were injected with 100 micrograms of colchicine (64868) and sacrificed. Liver, lung and bone marrow cells were isolated. Differential labeling of sister chromatids occurred simultaneously in alveolar macrophages regenerating liver and bone marrow cells of partially hepatectomized mice and in alveolar macrophages and bone marrow cells of nonhepatectomized mice. The percentage of second division cells depended on cell type, and no significant differences were apparent between hepatectomized and nonhepatectomized mice. BrdU incorporation did not significantly alter the mitotic yield or relative cell density of macrophage preparations. The authors note that this multicellular assay is useful in simultaneously assessing the relative mutagen induced effects in a variety of tissues.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Cellular-reactions; Chemical-analysis; Laboratory-animals; Chromosome-damage; Genetic-disorders; Reproductive-effects; Bioassays
Contact
Occupational Health University of Pittsburgh 130 DE Soto Street Pittsburgh, PA 15213
CODEN
CHROAU
CAS No.
59-14-3; 64-86-8
Publication Date
19790901
Document Type
Journal Article
Funding Amount
332059
Funding Type
Grant
Fiscal Year
1979
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-000367
Issue of Publication
1
ISSN
0009-5915
Priority Area
Pulmonary-system-disorders
Source Name
Chromosoma
State
PA
Performing Organization
University of Pittsburgh, Pittsburgh, Pennsylvania
TOP