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Metabolism of Azo Dyes to Potentially Carcinogenic Aromatic Amines.

Authors
Nony-CR
Source
National Center for Toxicological Research, Jefferson, Arkansas 1979 Mar:83 pages
Link
NIOSHTIC No.
00093822
Abstract
The in vivo transformation of Direct-Black-38 (1937377) (DB 38) and Pigment-Yellow-12 (6358856) (PY 12) to potentially carcinogenic metabolites was studied. Hamsters were given a single oral dose of 100 milligrams per kilogram of DB 38 or PY 12. Urine samples were collected for up to 7 days after treatment, and analyzed by electron capture gas chromatography and high pressure liquid chromatography. Urine samples from the animals treated with DB 38 were tested for mutagenicity in the Ames Salmonella microsome assay. Urine from the DB 38 treated hamsters contained benzidine (92875), monoacetylbenzidine (3366618), diacetylbenzidine (613354), 4- aminobiphenyl (92671), and alkaline hydrolyzable conjugates of benzidine and 4-aminobiphenyl. The greatest amounts of most of the metabolites were excreted within 8 hours of dosing; the greatest amounts of diacetylbenzidine and alkaline hydrolyzable conjugates of benzidine were excreted 8 to 16 hours after dosing. By 32 hours, most of the metabolite excretion returned to background levels, but monoacetylbenzide and alkaline hydrolyzable conjugates of benzidine were still detected 7 days after treatment. Mutagenic potential was correlated with the amount of monoacetylbenzidine present. None of the proposed metabolites of PY 12 were found in the urine of hamsters treated with that dye. The authors conclude that occupational exposure to DB 38 increases the risk of bladder cancer. Industrial discharge of the dye could result in environmental contamination. The use of this dye should be monitored.
Keywords
NIOSH-Contract; Contract-224-78-0004; Invivo-study; Laboratory-animals; Carcinogenesis; Subcellular-structure; Oxidative-metabolism; Sampling-methods; Chemical-exposure; Mutagenesis; Coloring-materials; Analytical-methods; Body-retention; Metabolic-effects;
CAS No.
1937-37-7; 6358-85-6; 92-87-5; 3366-61-8; 613-35-4; 92-67-1;
Publication Date
19790301
Funding Type
Contract;
Fiscal Year
1979
NTIS Accession No.
PB85-221398
NTIS Price
A06
Identifying No.
Contract-224-78-0004
Source Name
National Center for Toxicological Research, Jefferson, Arkansas 72079, 83 pages, 16 references
State
AR; KS;
Performing Organization
National Center for Toxicological Research Jefferson, Arkansas 72079
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