Intrahepatic mutagenesis assay: a sensitive method for detecting N- nitrosomorpholine and in vivo nitrosation of morpholine.
Edwards-G; Whong-WZ; Speciner-N
Mutat Res 1979 Dec; 64(6):415-423
An intrahepatic host mediated mutagenicity assay was developed to detect low concentrations of N-nitrosomorpholine (59892) (NMOR). Female mice were injected with a Salmonella typhimurium saline suspension, followed 10 minutes later by 0.2 milliliters (ml) of morpholine (110918) (MOR) and 0.2ml of sodium nitrite (7632000) by gavage. Control animals were intubated with nitrite alone, MOR alone, or only a citrate buffer solution. Animals were sacrificed at various intervals. Livers were removed, homogenized, and assayed. Mutagenicity was dose related. Bacterial survival under acidic conditions was not different between treated and control mice. Approximately 12 to 19 percent of the administered morpholine was converted to N-nitrosomorpholine (NMOR) when MOR was given together with nitrite. Nitrosation and the subsequent uptake and activation of the NMOR occurred rapidly; most of the total mutagenic response was complete within 15 minutes. No increased mutagenic activity was observed when MOR or nitrite was administered alone. The authors conclude that the intrahepatic mutagenicity assay is superior to intraperitoneal assays in detecting nitrosation of small doses of MOR. They suggest that this new mutagenicity assay could help determine whether ingestion of low levels of amines are potential health hazards.
NIOSH-Author; Bioassaying; Invivo-study; Invitro-study; Detection-equipment; Analytical-methods; Dose-responses; Sensitivity-tests
59-89-2; 110-91-8; 7632-00-0