The principles of the techniques of spectrophotometry and their application are reviewed. Included are terms used in spectrophotometry, such as theoretical background, visible light spectrophotometry, ultraviolet spectrophotometry, infrared spectrophotometry, fluoresence spectrophotometry, and atomic absorption spectrophotometry. Instrumentation and applications are given for each type of spectrophotometry. Schematic diagrams are given, including the electromagnetic spectrum, a filter photometer, a prism or grating spectrophotometer, a double beam spectrophotometer, a double beam infrared spectrophotometer, two types of spectrophotofluorometers and a basic atomic absorption spectrophotometer. Graphs of absorbance spectra include permanganate ion in water, dithizone in chloroform, lead dithizonate, benzene, toluene, benzene in cyclohexane, chlorinated hydrocarbons, and benzo(a)pyrene. Data are also given including the ultraviolet cutoff wavelength of common organic solvents, infrared absorption bands of compounds involved in environmental control work such as aromatic hydrocarbons and alkanes, spectral responses of some photomultiplier tubes, detection limits of the elements for atomic absorption spectrophotometry, and elements suitable for analysis by atomic absorption and flame photometry.
The Industrial Environment - Its Evaluation and Control, NIOSH, Cincinnati, Ohio, Chapter 19