Immunofluorescent studies in patients with farmer's lung.
Wenzel-FJ; Emanuel-DA; Gray-RL
J Allergy Clin Immunol 1971 Oct; 48(4):224-229
Immunofluorescent studies in patients with farmer's lung are described. Farmer's lung is one of the most important environmental agricultural diseases in the U.S. However, the immunologic process and its relationship to the pathophysiology of the class of diseases characterized by hypersensitivity pneumonitis are not clear. Pulmonary tissues from patients with farmer's lung have been studied with the use of fluorescein-labeled globulins from patients with the disease and with fluorescein-labeled antisera. Immunoglobulins were found in the plasma cells and lymphocytes. The walls of the bronchioles appeared rich in antigen, staining well with the fluorescein-labeled globulins isolated from patients with the disease. One immunoglobulin was presented in the histiocytes, suggesting the antecedent presence of antigen-antibody complexes. No histologic or immunologic evidence of vasculitis could be found. The clinical findings suggested further investigation of the hypothesis that at least part of the pathogenesis of farmer's lung may involve a cytotoxic reaction. In this event, antigen adsorbed to the cells reacts with antibody in the presence of complement, causing cellular destruction. The presence or absence of a delayed component is still uncertain.
NIOSH-Grant; Immunology; Cytopathology; Fluorescence; Cytotoxicity; Etiology; Histopathology
Internal Medicine Marshfield Clinic Foundation 510 North St Joseph Ave Marshfield, Wis 54449
Journal of Allergy and Clinical Immunology (Paper presented at the Annual Meeting of the American Academy of Allergy, February 23, 1971)
Marshfield Clinic Fdn Med Res Education, Marshfield, Wisconsin