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Fractionation and chemical characterization studies on Micropolyspora faeni antigens.

Authors
Roberts-RC
Source
Ann NY Acad Sci 1974 Feb; 221(1):199-204
NIOSHTIC No.
00041586
Abstract
Fractionation and chemical characterization studies on Micropolyspora faeni antigens are reported. Persons with hypersensitivity pneumonias such as farmer's lung usually show precipitating antibodies to antigens such as this. Two new isolation procedures were used. First the antigen mixture obtained by double dialysis was fractionated by ammonium-sulfate (7783202). Second, affinity chromatography using immobilized antibodies was employed. The chemical data on the 3 components fractionated with ammonium- sulfate are given (percent dryweight, percent amino acids, percent carbohydrate). The use of immobilized antibodies allows determination of inert as well as antigenically active material.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders
Contact
Internal Medicine Marshfield Clinic Foundation 510 North St Joseph Ave Marshfield, Wis 54449
CODEN
ANYAA9
CAS No.
7783-20-2
Publication Date
19740201
Document Type
Journal Article
Funding Amount
104470
Funding Type
Grant
Fiscal Year
1974
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00306
Issue of Publication
1
ISSN
0077-8923
Priority Area
Pulmonary-system-disorders
Source Name
Annals of the New York Academy of Sciences
State
WI
Performing Organization
Marshfield Clinic Fdn Med Res Education, Marshfield, Wisconsin
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