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Specific metal staining of biological tissues for the SEM using backscatter electron or specimen current imaging.

Authors
Abraham-JL; DeNee-PB; Gelderman-AH
Source
NIOSH 1973 Aug; :1-4
Link
NIOSHTIC No.
00024962
Abstract
A technique is described for distinguishing specific cell and tissue components with the Scanning Electron Microscope, combining differential impregnation of tissue by heavy elements with the backscatter mode of SEM imaging. Advantages of the method include large depth of field, surface morphology, histochemical identification of structures, and high resolution, with no special tissue processing required. Illustrations are presented to demonstrate the potential usefulness of the technique in investigations of coal workers' pneumoconiosis.
Keywords
Analytical-methods; Electron-microscopy; Microscopy; Electron-optics; Heavy-metals; Biologic-tissues; Respiratory-system-disorders
Publication Date
19730801
Document Type
Other
Fiscal Year
1973
NTIS Accession No.
NTIS Price
NIOSH Division
ALFORD
Priority Area
Respiratory-system-disorders
Source Name
Appalachian Laboratory for Occupational Respiratory Diseases, NIOSH (Reprint, Electronic Society of America, August, 1973), Morgantown, West Virginia, 4 pages, 5 references
State
VA; WV
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