In vitro lymphocyte responses in contact hypersensitivity II.
J Invest Dermatol 1971 May; 56(5):349-352
To determine whether lymphocyte transformation in-vitro in response to hapten skin protein conjugates is specific as well as sensitive, guinea-pigs were made sensitive to either of two contact allergens, dinitrofluorobenzene (70348) and p-phenylenediamine (106503). Sensitization was accomplished by footpad injections of the conjugated hapten. Lymphocytes from animals with contact hypersensitivity to the two allergens were cultured in-vitro with conjugates of guinea-pig epidermal proteins with the same allergens. Responses to these antigens indicate that lymphocyte transformation in-vitro, in the presence of the appropriate conjugate, can distinguish between sensitivity to either of these antigens.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Allergic-reactions; Skin-disorders; Immunology; White-blood-cells; Amines; Fluorinated-benzenes
Environmental Health University of Washington Dept of Environ Health Seattle, Wash 98105
Journal of Investigative Dermatology
University of Washington, Seattle, Washington