M.R. Gwinn, D.L. Whipkey,
A. Weston, |
Corresponding
author: Ainsley Weston, Ph.D. NIOSH, 1095 Willowdale Road, Morgantown WV 26505 Tel: 304-285-6126; Fax: 304-285-6038 |
Table
1. Genes Increased in 3 or
more strains at least one time point |
||
GenBank
ID |
Name |
Function |
| U22028 | CYP2A13 | chemical metabolism |
| U20734 | junB | cell proliferation |
| V01512 | cfos | cell proliferation |
| S85655 | prohibitin | inhibit DNA synthesis |
| S82240 | RhoE GTPase | Ras-like protein |
| X83492 | fas/Apo1 | apoptosis |
| X66899 | EWS | oncogenesis |
| M63573 | cyclophilin | immunosuppression |
| AB000450 | VRK2 | serine/threonine kinase |
| S73591 | VDUP1 | brain protein homolog |
| X52541 | early growth response | |
| AB000464 | Clone | unknown |
| D42073 | reticulocalbin | calcium binding protein |
| M37766 | MEM-102 glycoprotein | HLA CD48 |
| Table 2. Genes Decreased in 3 or more strains at least one time point | ||
| GenBank ID | Name | Function |
| L05624 | MAP Kinase Kinase | signal transduction |
| U64520 | Synaptobrevin-3 | fusion protein |
| U23435 | ABI interactor 2 | tumor suppressor |
| M20902 | Apoliprotein C-I | lipid metabolism |
| U18018 | E1A enhancer | oncogenesis |
| S72043 | GIF | growth inhibition factor |
| J02783 | thyroid hormone binding | proline hydroxylation |
| J04029 | keratin 10 type I | epidermal differentiation |
| M27436 | tissue factor gene | immune response |
| U04241 | Dros. enhancer split m9/m10 | developmental processes |
| U40282 | integrin-linked kinase | integrin receptor signaling |
| U62015 | Cyr61 | cell proliferation |
| X06272 | docking protein | membrane targeting |
| X52979 | SmB and SmB' | mRNA splicing |
| X56681 | junD | transcriptional regulation |
| X64364 | M6 antigen | signal transduction |
| X76040 | Lon protease-like protein | proteolysis |
| X77366 | HBZ17 | inflammatory response |
| X86809 | PEA15 | small molecule transport |
| X98801 | dynactin | mitosis |
| M11717 | HSP70 | inflammation |
| U16799 | Na, K-ATPase beta1 subunit | |
| X68836 | S-adenosylmethionine synthetase | |
| X99209 | arginine methyltransferase | |
| Table 3. Genes Increased in Major Variant after OTQ exposure as compared to Control | ||
| GenBank ID | Name | Function |
| L42025 | RAB | nuclear export |
| U63717 | osteoclast | bone development |
| U62325 | FE65-like | unknown |
| X03484 | raf oncogene | MAP3K pathway |
| Z50194 | PQ-rich (liver) | unknown |
| M73239 | hepatocyte growth factor | liver development |
| D86988 | Human mRNA for KIAA0221 gene | RNA processing |
| L10910 | splicing factor (CC1.3) | RNA processing |
| L28010 | HnRNP F protein | RNA processing |
| M27281 | vascular permeability factor | positive control of cell proliferation |
| M54915 | h-pim-1 protein (h-pim-1) | protein phosphorylation |
| M57731 | Human gro-beta | chemotaxis, inflammatory response |
| M60974 | growth arrest and DNA-damage-inducible protein (gadd45) | DNA repair, cell cycle arrest, regulation of CDK activity |
| M69043 | MAD-3 mRNA encoding IkB-like activity | apoptosis |
| M83667 | NF-IL6-beta protein | transcription from Pol II promoter |
| M95809 | basic transcription factor 62kD subunit (BTF2) | DNA repair, regulation of CDK activity |
| U15932 | Human dual-specificity protein phosphatase | protein dephosphorylation |
| U26173 | Human bZIP protein NF-IL3A (IL3BP1) | immune response |
| U28833 | Human Down syndrome critical region protein (DS | signal transduction |
| U31501 | fragile X mental retardation syndrome related protei | pathogenesis |
| U31556 | transcription factor E2F-5 | cell cycle control |
| U31930 | deoxyuridine nucleotidohydrolase | nucleobase, nucleoside, nucleotide and nucleic acid metabolism |
| U41740 | trans-Golgi p230 | vesicle transport |
| U44754 | PSE-binding factor PTF gamma subunit | transcription from Pol III promoter |
| U62325 | FE65-like protein (hFE65L) | protein phosphorylation |
| U72649 | BTG2 | DNA damage response, activation of p53, DNA repair |
| U79270 | Human clone 23707 | cytochrome c oxidase biogenesis |
| X04500 | prointerleukin 1 beta | negative control of cell proliferation |
| X57579 | activin beta-A subunit (exon 2) | cell-cell signalling, cell growth and maintenance |
| X65488 | U21.1 | RNA processing |
| X69878 | Flt4 mRNA for transmembrane tyrosine kinase | transmembrane receptor protein tyrosine kinase signalling pathway |
| X78565 | tenascin-C | cell adhesion |
| X83416 | PrP gene, exon 2 | virulence |
| X85753 | CDK8 protein kinase | cell cycle control |
| X89750 | TGIF protein | repression of transcription from Pol II promoter |
| X97065 | Sec23B isoform | vesicle transport |
| Table 4. Genes Decreased in Major Variant after OTQ exposure as compared to Control | ||
| GenBank ID | Name | Function |
| AB000449 | VRK1(liver) | serine/threonine Kinase |
| M24349 | PLP gene | parathyroid hormone-like |
| L19871 | ATF3 | transcription control |
| S78234 | nuc2 homolog | retinoblastoma interaction |
| U46006 | smooth muscle LIM protein | |
| L37378 | guanylyl cyclase (RetGC-2) | receptor guanylyl cyclase signalling pathway |
| Z78289 | clone 1D2 | |
| S81243 | steroid/thyroid orphan receptor homolog gene | EWS? |
| D16815 | EAR-1r | signal transduction |
| M61156 | Human activator protein 2B (AP-2B) | oncogenesis |
| L76687 | Grb14 | signal transduction |
| U90065 | potassium channel KCNO1 | potassium transport |
| X56677 | MyoD | transcription regulation from Pol II promoter |
| D13264 | macrophage scavenger receptor type I | |
| X59618 | RR2 mRNA for small subunit ribonucleotide reductase | |
| M65066 | cAMP-dependent protein kinase regulatory subunit RI-beta | |
| J04132 | T cell receptor zeta-chain | cellular defense response |
| U68727 | homeobox-containing protein | |
| M31520 | ribosomal protein S24 | |
| X79780 | YPT3 | |
| Z84722 | Human DNA sequence from cosmid GG4 from a contig from the tip of the short arm of chromosome 16, spanning 2Mb of 16p13.3. | |
| X68090 | Fc-gamma-RIIA gene for IgG Fc receptor class IIA | immune response |
| U79246 | Human clone 23799 | |
| U82256 | arginase type II | arginine catabolism |
| X16546 | eosinophil derived neurotoxin | immune response |
| J04513 | basic fibroblast growth factor (bFGF) | |
| M76125 | tyrosine kinase receptor (axl) | cell cycle control |
| Y14140 | G protein gene encoding beta 3 subunit exon 1 and promoter | |
| S73840 | type IIx myosin heavy chain | |
| M64497 | Human apolipoprotein AI regulatory protein (ARP-1) | transcription regulation from Pol II promoter |
| Y07512 | type I beta cGMP-dependent protein kinase | actin cytoskeleton reorganization |
| M94055 | voltage-gated sodium channel | sodium transport |
| Z83806 | axonemal dynein heavy chain | |
| D14823 | Human chimeric mRNA derived from AML1 gene and MTG8(ETO) gene, partial sequence. | |
| U82979 | immunoglobulin-like transcript-3 | signal transduction |
| D86985 | KIAA0232 | |
| U68019 | mad protein homolog (hMAD-3) | TGFbeta receptor signalling pathway |
| Z69881 | adenosine triphosphatase | small molecule transport |
| U50360 | calcium, calmodulin-dependent protein kinase II gamma | |
| U39231 | GIP receptor (GIPR) | cell surface receptor linked signal transduction |
| Cyclin D1 Promoter | ||
| M58583 | precerebellin and cerebellin | synaptic transmission |
| S85963 | hIRS-1=rat insulin receptor substrate-1 homolog | signal transduction |
| X59372 | HOX4C for a homeobox protein | |
| S78723 | serotonin 5-HT2A receptor {promoter} | |
| X76342 | ADH7 | |
| D12775 | erythrocyte-specific AMP deaminase | AMP catabolism |
| X95677 | ArgBPIB protein | |
| L07590 | protein phosphatase 2A 130 kDa regulatory subunit | |
| Table 5. Genes Increased in Intermediate Variant after OTQ exposure as compared to Control | ||
| GenBank ID | Name | Function |
| AB000114 | Osteomodulin | bone development |
| AB000115 | Osteoblast | bone development |
| L78833 | BRCA1-related | unknown |
| U42031 | Immunophilin | progesterone-receptor |
| AB000905 | H4 Histone | |
| AB001106 | glia maturation | |
| AF005037 | SCAMPI | |
| D12625 | NF1 isoform | |
| D12676 | lysosomal | |
| M16653 | pancreatic elastase | |
| M55422 | HPLK | Kruppel? |
| U18543 | zinc finger | |
| X59244 | ZNF43 | Kruppel-related |
| U18321 | Human ionizing radiation resistance conferring prot | induction of apoptosis by extracellular signals |
| U14575 | ard-1 | RNA catabolism |
| L27624 | tissue factor pathway inhibitor-2 | |
| X70340 | transforming growth factor alpha | cell proliferation |
| N-Ethylmaleimide-Sensitive Factor | ||
| M24470 | glucose-6-phosphate dehydrogenase | nucleobase, nucleoside, nucleotide and nucleic acid metabolism |
| M26041 | MHC class II DQ alpha | pathogenesis |
| M34458 | lamin B | |
| U65406 | KCNJ1 gene (potassium channel ROM-K3) extrac | potassium transport and excretion |
| U31201 | laminin gamma2 chain gene (LAMC2) | |
| Z36715 | Net transcription factor | signal transduction |
| U79274 | Human clone 23733 | unknown |
| U59289 | H-cadherin | cell adhesion |
| U09087 | thymopoietin beta | |
| Z24724 | H.sapiens polyA site DNA | |
| U28749 | high-mobility group phosphoprotein isoform I-C (HMGIC) | chromatin architecture and transcription regulation |
| Z95624 | Human DNA sequence from cosmid U237H1 contains Ras like GTPase and ESTs. | |
| U04840 | onconeural ventral antigen-1 (Nova-1) | RNA splicing |
| U85658 | transcription factor ERF-1 | transcription regulation from Pol II promoter |
| X14008 | lysozyme gene | |
| D80003 | Human mRNA for KIAA0181 gene | |
| X53586 | integrin alpha 6 (or alpha E) protein gene | cell-substrate junction assembly |
| L40393 | Homo sapiens (clone S171) mRNA | |
| X82200 | Staf50 | transcription regulation |
| U85193 | Human nuclear factor I-B2 (NFIB2) | |
| M31523 | transcription factor (E2A) | oncogenesis |
| D31891 | Human mRNA for KIAA0067 gene | unknown |
| Z29064 | AF-1p | cell proliferation and oncogenesis |
| M37197 | Human CCAAT-box-binding factor (CBF) | transcription from Pol II promoter |
| X14787 | thrombospondin | developmental processes |
| U72263 | multiple exostoses type II protein EXT2.I | glycosaminoglycan biosynthesis |
| X81625 | Cl1 protein | translational regulation, termination |
| D87120 | cancellous bone osteoblast | |
| D83243 | NPAT | |
| Estrogen Sulfotransferase, Ste | ||
| X54326 | glutaminyl-tRNA synthetase | protein complex assembly |
| D38521 | Human mRNA for KIAA0077 gene | unknown |
| U88666 | serine kinase SRPK2 | RNA splicing |
| D38073 | hRlf beta subunit (p102 protein) | DNA replication initiation |
| X69433 | mitochondrial isocitrate dehydrogenase (NADP+) | carbohydrate metabolism |
| U26424 | Ste20-like kinase (MST2) | heat shock response |
| L47276 | alpha topoisomerase truncated-form | |
| M97935 | transcription factor ISGF-3 | caspase act.,cell cycle control, NIK-I-kappaB/NF-kappaB,JAK-STAT cascade |
| J00073 | alpha-cardiac actin gene | |
| M38258 | retinoic acid receptor gamma 1 | developmental processes |
| D78611 | MEST | mesoderm development |
| X52947 | cardiac gap junction protein | cell-cell signalling |
| U50196 | adenosine kinase | ribonucleoside monophosphate biosynthesis |
| M19267 | tropomyosin | control of heart |
| L20859 | leukemia virus receptor 1 (GLVR1) | small molecule transport and phosphate metabolism |
| X78627 | translin | DNA recombination |
| D50405 | RPD3 protein | |
| X81198 | archain | intracellular protein traffic |
| M82882 | Human cis-acting sequence | |
| X99586 | SMT3C protein | small ubiquitin-related protein 1 conjugation |
| U44975 | Human DNA-binding protein CPBP | |
| D29992 | placental protein 5 (PP5) | |
| Y00264 | amyloid A4 precursor of Alzheimer disease | cell death |
| Z68204 | succinyl CoA synthetase | |
| M34192 | isovaleryl-coA dehydrogenase (IVD) | |
| Z29678 | mitF | |
| X55544 | TREB protein | |
| U22431 | hypoxia-inducible factor 1 alpha (HIF-1 alpha) | transcription, DNA-dependent |
| U00943 | Human clone A9A2BRB2 (CAC)n/(GTG)n repeat-c | unknown |
| M29273 | myelin-associated glycoprotein (MAG) | |
| X62534 | HMG-2 mRNA | |
| X75304 | giantin | Golgi organization and biogenesis |
| U18271 | thymopoietin (TMPO) gene | |
| Table 6. Genes Decreased in Intermediate Variant after OTQ exposure as compared to Control | ||
| GenBank ID | Name | Function |
| J04973 | Cytochrome bc-1 | mito. core protein |
| L18960 | eIF-4C | protein synthesis |
| M32886 | Sorcin CP-22 | calcium binding |
| U05861 | Dihydrodiol dehyd. | hepatic enzyme |
| U20428 | SNC19 | unknown |
| U32519 | GAP SH3 Binding | ras-GTPase-activating |
| D14663 | clone | unknown |
| D29958 | clone | unknown |
| M22632 | aspartate aminotransferase | aspartate catabolism |
| X99585 | arginin metab. | |
| Z69720 | ESTs | unknown |
| M26311 | cystic fibrosis antigen | cell-cell signaling |
| M60047 | heparin binding protein | cell proliferation |
| M88108 | p62 | cell cycle arrest |
| U09813 | mitochondrial ATP synthase | energy pathways |
| U14588 | paxillin | cell adhesion |
| U53204 | plectin | cytoskeletal anchoring |
| U67122 | ubiquitin-related SUMO-1 | DNA repair |
| U73379 | cyclin-selective ubiquitin | cyclin degradation |
| U78525 | eIF3 | protein synthesis |
| U85611 | DNA-PK interaction (KIP) | double-strand break repair |
| X04654 | U1 RNA associated protein | mRNA processing |
| X52425 | IL-4 receptor | signal transduction |
| X64330 | ATP-citrate lyase | ATP catabolism |
| Z47055 | farnesyl pyrophosphate synthetase-like | unknown |
| U79287 | clone | unknown |
Table 7. All Microarray Data Following Oxythioquinox Exposure
Table 7 _1
Table 7_2
Table 7_3