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Registry of Toxic Effects of Chemical Substances (RTECS)

Toluene, 2,4-dinitro-

RTECS #

XT1575000

CAS #

121-14-2

Updated

December 2012

Molecular Weight

182.15

Molecular Formula

C7H6N2O4

Synonyms

1-Methyl-2,4-dinitrobenzene
2,4-Dinitrotoluene
2,4-Dinitrotoluol
2,4-DNT
4-Methyl-1,3-dinitrobenzene
Benzene, 1-methyl-2,4-dinitro-
Dinitrotoluene (OSHA)
DNT
NCI-C01865
RCRA waste number U105

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
skin /rabbit 500 mg/24H mildNTIS** AD-B011-150

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisoral/rat4200 mg/kg/5W-continuousMUREAV 38,387,1976
DNA adductoral/rat10 mg/kgJTEHD6 11,555,1983
DNA Damageliver/rat3 mmol/LSinJF# 26OCT1982
DNA Damagetestis/rat0.032 µmol/LEMMUEG 44,179,2004
DNA inhibitionintraperitoneal/rat20 mg/kg/8W- intermittentCBTOE2 7,111,1991
Dominant Lethal Testoral/rat10920 mg/kg/13W-continuousNTIS** AD-A061-715
micronucleus testoral/rat150 mg/kg/2D- intermittentMUREAV 698,24,2010
morphological transformoral/rat567 mg/kg/3W-continuousCRNGDP 7,1797,1986
mutation in mammalian somatic cellsliver/rat600 µmol/LBANRDU 2,303,1979
mutation in mammalian somatic cellslymphocyte/mouse200 mg/LCALEDQ 20,103,1983
mutation in microorganisms/Salmonella typhimurium313 µg/plate/20M (-enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium625 µg/plate/20M (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium625 µg/plate/48H (-enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium1250 µg/plate/48H (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium10 µg/plate (+enzymatic activation step)NTIS** AD-A080-146
mutation in microorganisms/Salmonella typhimurium125 µg/plate (-enzymatic activation step)ENMUDM 7(Suppl 5),1,1985
other mutation test systems/Salmonella typhimurium50 mg/LMUREAV 272,91,1992
other mutation test systemsoral/rat10 mg/kgJTEHD6 11,555,1983
sex chromosome loss and nondisjunctionparenteral/Drosophila melanogaster200 ppmENMUDM 7,677,1985
sister chromatid exchangeovary/hamster1010 mg/LEMMUEG 13,60,1989
Unscheduled DNA Synthesisother cell types/rat10 µmol/LCBTOE2 7,111,1991
Unscheduled DNA Synthesisoral/rat35 mg/kgEMMUEG 14,155,1989

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/rat 3094 mg/kg (13W male)Reproductive: Effects on fertility: Pre- implantation mortality (e.g., reduction in number of implants per female; total number of implants per corpora lutea)

Reproductive: Effects on fertility: Other measures of fertility
NTIS** AD-A077-692
oral/rat 8463 mg/kg (13W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)JACTDZ 4(4),243,1985
oral/rat 2380 mg/kg (70D male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands
REPTED 28,196,2009
oral/rat 7.3 gm/kg (70D male/70D prior to copulation/22D pregnant)Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive)REPTED 28,196,2009

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 10080 mg/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Kidney tumors
JACTDZ 4(4),257,1985
oral/mouse toxic dose: 8760 mg/kg/2Y- continuousTumorigenic: Neoplastic by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors

Kidney, Ureter, and Bladder: Kidney tumors
NCITR* NCI-TR-54,1978
oral/rat lowest published toxic dose: 2620 mg/kg/78W- continuousTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors
NCITR* NCI-TR-54,1978
oral/rat toxic dose: 5460 mg/kg/78W- continuousTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors
NCITR* NCI-TR-54,1978
oral/rat toxic dose: 28 gm/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
NTIS** AD-A080-146
oral/rat toxic dose: 12775 mg/kg/1Y- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Liver: Tumors
PAACA3 24,91,1983

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
oral/domestic bird lethal dose (50 percent kill): 55 mg/kgBehavioral: Sleep

Behavioral: Fluid intake

Gastrointestinal: Hypermotility, diarrhea
IJTOFN 24,265,2005
oral/mouse lethal dose (50 percent kill): 790 mg/kgGTPZAB 25(8),50,1981
oral/rat lethal dose (50 percent kill): 268 mg/kgNTIS** PB214-270
skin/guinea pig lethal dose (50 percent kill): >1 gm/kgNTIS** OTS0572393
subcutaneous/cat lowest published lethal dose: 25 mg/kgXPHBAO 271,110,1941

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/Dog lowest published toxic dose: 2250 mg/kg/90D- intermittentBlood: Normocytic anemia

Blood: Methemoglobinemia- Carboxhemoglobinemia

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
TXAPA9 37,116,1976
oral/Dog lowest published toxic dose: 7280 mg/kg/2Y- intermittentBrain and Coverings: Other degenerative changes

Blood: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** AD-A080-146
oral/domestic bird lowest published toxic dose: 490 mg/kg/14D- intermittentLiver: Changes in liver weight

Kidney, Ureter, and Bladder: Changes in kidney weight
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 60 mg/kg/4D- intermittentBehavioral: Sleep

Gastrointestinal: Hypermotility, diarrhea

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 75 mg/kg/3D- intermittentBehavioral: Sleep

Gastrointestinal: Hypermotility, diarrhea

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 900 mg/kg/60D- intermittentBrain and Coverings: Changes in brain weight

Kidney, Ureter, and Bladder: Changes in kidney weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 900 mg/kg/60D- intermittentBrain and Coverings: Changes in brain weight

Kidney, Ureter, and Bladder: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 1500 mg/kg/60D- intermittentReproductive: Maternal effects: Ovaries, fallopian tubes

Related to Chronic Data: Changes in ovarian weight
IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 300 mg/kg/60D- intermittentKidney, Ureter, and Bladder: Changes in kidney weightIJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 300 mg/kg/60D- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)IJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 60 mg/kg/60D- intermittentBlood: Other changesIJTOFN 24,265,2005
oral/domestic bird lowest published toxic dose: 300 mg/kg/60D- intermittentKidney, Ureter, and Bladder: Other changes

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
IJTOFN 24,265,2005
oral/mouse lowest published toxic dose: 37583 mg/kg/13W- continuousBlood: Normocytic anemia

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JACTDZ 4(4),257,1985
oral/mouse lowest published toxic dose: 654 mg/kg/2Y- continuousBlood: Normocytic anemia

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in other cell count (unspecified)
JACTDZ 4(4),257,1985
oral/mouse lowest published toxic dose: 328 gm/kg/1Y- continuousEndocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight
NTIS** AD-A077-692
oral/rat lowest published toxic dose: 273 mg/kg/70D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainREPTED 28,196,2009
oral/rat lowest published toxic dose: 2380 mg/kg/70D- intermittentRelated to Chronic Data: Changes in testicular weightREPTED 28,196,2009
oral/rat lowest published toxic dose: 3150 mg/kg/70D- intermittentRelated to Chronic Data: Changes in ovarian weightREPTED 28,196,2009
oral/rat lowest published toxic dose: 1260 mg/kg/3W- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainTXAPA9 94,466,1988
oral/rat lowest published toxic dose: 2520 mg/kg/3W- continuousEndocrine: Change in LH

Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
TXAPA9 94,466,1988
oral/rat lowest published toxic dose: 13195 mg/kg/13W- continuousBehavioral: Food intake (animal)

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JACTDZ 4(4),243,1985
oral/rat lowest published toxic dose: 24752 mg/kg/2Y- continuousBlood: Normocytic anemia

Blood: Changes in other cell count (unspecified)
JACTDZ 4(4),243,1985
oral/rat lowest published toxic dose: 12410 mg/kg/1Y- continuousBlood: Normocytic anemia

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight
NTIS** AD-A077-692
oral/rat lowest published toxic dose: 76 gm/kg/26W- continuousLiver: Changes in liver weight

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Related to Chronic Data: Changes in testicular weight
JTSCDR 4,221,1979

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 65,309,1996
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 65,309,1996
International Agency for Research on Cancer (IARC)Cancer Review:Group 2BIMEMDT 65,309,1996
TOXICOLOGY REVIEWNTIS** AD778-725
TOXICOLOGY REVIEWMUREAV 543,201,2003
TOXICOLOGY REVIEWMUREAV 567,227,2004
TOXICOLOGY REVIEWMUREAV 567,347,2004
TOXICOLOGY REVIEWMUREAV 588,88,2005
TOXICOLOGY REVIEWMUREAV 627,10,2007
TOXICOLOGY REVIEWENTOX* -,60,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWBCLPT* 96,131,2005
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWMUTAEX 26,223,2011

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-DENMARKtime-weighted average 0.15 mg/m3, skin, carc, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 0.2 mg/m3, skin, NOV2011
Occupational Exposure Limit-ICELANDtime-weighted average 0.15 mg/m3, skin, NOV2011
Occupational Exposure Limit-JAPAN2B carc, MAY2012
Occupational Exposure Limit-MEXICOtime-weighted average 1.5 mg/m3;short term exposure limit 5 mg/m3 (skin), 2004
Occupational Exposure Limit-PERUtime-weighted average 0.15 mg/m3, JUL2005
Occupational Exposure Limit-RUSSIAtime-weighted average 1 mg/m3, short term exposure limit 3 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWEDENtime-weighted average 0.15 mg/m3;short term exposure limit 0.3 mg/m3, Carcinogen, Skin, JUN2005
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 1.5 mg/m3 (skin)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 1.5 mg/m3 (skin)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 1.5 mg/m3 (skin)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 1.5 mg/m3 (skin)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO 2,4-DINITROTOLUENE-air10H Carcinogen time-weighted average 1.5 mg/m3 (Sk)NIOSH* DHHS #92-100,1992

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/99/122004)
EPA GENETOX PROGRAM 1988, Inconclusive: CHO gene mutation
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);no evidence:mouseNCITR* NCI-TR-54,1978
NCI Carcinogenesis Bioassay (feed);some evidence:ratNCITR* NCI-TR-54,1978
NIOSH CURRENT INTELLIGENCE BULLETIN #44
On EPA IRIS database
OSHA ANALYTICAL METHOD #44
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