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Registry of Toxic Effects of Chemical Substances (RTECS)

Sulfuric acid, diethyl ester

RTECS #

WS7875000

CAS #

64-67-5; 98503-29-8

Updated

July 2013

Molecular Weight

154.20

Molecular Formula

C4H10O4S

Synonyms

Diaethylsulfat (German)
Diethyl sulfate
Diethyl sulphate
Diethyl tetraoxosulfate
Diethylester kyseliny sirove (Czech)
Ethyl sulfate

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 23.6 mg severeHPV392 -,-,2004
skin /rabbit 500 mg open irritation test mildUCDS** 12/30/1971
skin /rabbit 11 mgHPV392 -,-,2004

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysislymphocyte/human300 µmol/LMUREAV 205,409,1988
Cytogenetic Analysisintraperitoneal/mouse150 mg/kgMUREAV 75,63,1980
Cytogenetic Analysisovary/hamster1297 µmol/LMUTAEX 4,432,1989
Cytogenetic Analysisovary/hamster0.04 pph/5H (+/-enzymatic activation step)HPV392 ,,2004
DNA adductlymphocyte/mammal (species unspecified)100 mmol/LBIJOAK 80,496,1961
DNA adductAscites tumor/mouse5 µmol/LMUREAV 89,95,1981
DNA adduct/Escherichia coli5 µmol/LMUREAV 89,95,1981
DNA Damage/Other microorganisms9 gm/LCRNGDP 2,363,1981
DNA Damageintraperitoneal/rat39 mg/kgTCMUD8 7,175,1987
DNA DamageHeLa cell/human20 mgBICHAW 14,1795,1975
DNA Damageintraperitoneal/mouse250 mg/kgMUREAV 467,83,2000
DNA Damageovary/hamster2500 µmol/LMUREAV 92,361,1982
DNA Damagemammary gland/human21 mg/L/24H (-enzymatic activation step)MUREAV 588,47,2005
DNA Damage/Saccharomyes cerevisiae590 mg/L/17HMUREAV 633,80,2007
DNA Damagesperm/cattle1 mmol/L/120MREPTED 30,44,2010
DNA inhibitionintraperitoneal/mouse200 mg/kgMUREAV 46,305,1977
DNA inhibitionHeLa cell/human600 µmol/LMUREAV 92,427,1982
Dominant Lethal Testparenteral/silkworm100 µmol/kgNISZAQ 46,205,1977
Dominant Lethal Testintraperitoneal/mouse100 mg/kg/10W- intermittentGNKAA5 12(3),163,1976
Dominant Lethal Testparenteral/mouse150 mg/kg28QFAD -,97,1973
Dominant Lethal Testunreported route/mouse150 mg/kgSOGEBZ 10,1004,1974
gene conversion and mitotic recombination/Saccharomyes cerevisiae20 mmol/LZEVBA5 98,230,1966
heritable translocation testmultiple/Drosophila melanogaster5000 ppm/3.5H-continuousMUREAV 45,355,1977
host-mediated assaySalmonella typhimurium/mouse2500 µmol/kgCBINA8 19,241,1977
micronucleus testlung/hamster250 µmol/LMUREAV 205,409,1988
micronucleus testintraperitoneal/mouse16 mg/kg/48H- intermittentMUREAV 389,3,1997
micronucleus testmultiple/non-mammalian species6 ppm/6D-continuousMUREAV 164,245,1986
micronucleus testlymphocyte/hamster1 mmol/LMUREAV 205,409,1988
micronucleus testovary/hamster6 mmol/L/2HMUTAEX 18,405,2003
micronucleus testfibroblast/hamster6 mmol/L/2HMUTAEX 18,405,2003
micronucleus testovary/hamster385 mg/L/24HMUREAV 633,80,2007
micronucleus testintraperitoneal/mouse400 mg/kgHPV392 ,,2004
micronucleus testintraperitoneal/mouse320 mg/kg/2D- intermittentHPV392 ,,2004
mutation in mammalian somatic cellsovary/hamster1 mmol/LMUREAV 57,217,1978
mutation in mammalian somatic cellslung/hamster1 mg/L/1HCRNGDP 1,175,1980
mutation in microorganisms/Salmonella typhimurium1000 µg/plate/8H (-enzymatic activation step)HPV392 ,,2004
mutation in microorganisms/Neurospora crassa5000 ppm/40M (-enzymatic activation step)GENTAE 63,281,1969
mutation in microorganisms/Saccharomyes cerevisiae5000 ppm/15M (-enzymatic activation step)GENTAE 78,1101,1974
mutation in microorganisms/Aspergillus nidulans50 mmol/L (-enzymatic activation step)RBGED3 9,393,1986
mutation in microorganisms/Schizosaccharomyces pombe15300 µmol/L (-enzymatic activation step)CBINA8 14,195,1976
mutation in microorganisms/Bacillus subtilis5 mmol/L (-enzymatic activation step)EXPEAM 39,530,1983
mutation in microorganisms/Serratia marcescens2 gm/L (-enzymatic activation step)ATXKA8 28,93,1971
mutation in microorganisms/Other microorganisms3500 mg/L (-enzymatic activation step)PHABDI 18,165,1978
mutation in microorganisms/Salmonella typhimurium1 µL/plate (+enzymatic activation step)MUREAV 130,79,1984
mutation in microorganisms/Salmonella typhimurium5 mg/plate (-enzymatic activation step)MUREAV 57,141,1978
mutation in microorganisms/Escherichia coli10 mmol/L (-enzymatic activation step)JOBAAY 79,464,1960
other mutation test systems/Escherichia coli30 nmol/tubePNASA6 79,5971,1982
other mutation test systems/Salmonella typhimurium200 µg/LMUREAV 192,239,1987
phage inhibition capacity/Escherichia coli65 mmol/L/20MPRLBA4 150,497,1959
sex chromosome loss and nondisjunctionoral/Drosophila melanogaster1000 ppm/24HMUREAV 143,219,1985
sex chromosome loss and nondisjunctionmultiple/Drosophila melanogaster5000 ppmMUREAV 110,49,1983
sex chromosome loss and nondisjunctionlymphocyte/human100 µmol/LMUREAV 205,409,1988
sister chromatid exchangelung/hamster1 gm/LCNREA8 44,3270,1984
sister chromatid exchangeovary/hamster0.01 pph/5H (-enzymatic activation step)HPV392 ,,2004
specific locus testoral/Drosophila melanogaster10 mmol/L/3HMUREAV 545,59,2004
specific locus testintraperitoneal/mouse100 mg/kg/10WMUREAV 75,63,1980
specific locus testoral/Drosophila melanogaster10 mmol/LMUREAV 182,243,1987
specific locus testparenteral/silkworm30 ng/kgKIKNAJ (25),42,1974
Unscheduled DNA Synthesisliver/rat100 µmol/LENMUDM 3,11,1981
Unscheduled DNA Synthesisliver/rat0.0001 pph/2HHPV392 ,,2004

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intravenous/rat 340 mg/kg (15D pregnant)Reproductive: Effects on embryo or fetus: Fetal deathIARCCD 4,45,1973

Tumorigenic Data and References

Route/OrganismDoseEffectReference
intravenous/rat lowest published toxic dose: 85 mg/kg (15D pregnant)Tumorigenic: Carcinogenic by RTECS criteria

Reproductive: Tumorigenic effects: Transplacental tumorigenesis

Brain and Coverings: Tumors
IARCCD 4,45,1973
intravenous/rat lowest published toxic dose: 85 mg/kgTumorigenic: Neoplastic by RTECS criteria

Brain and Coverings: Tumors

Reproductive: Tumorigenic effects: Transplacental tumorigenesis
HPV392 -,-,2004
oral/rat lowest published toxic dose: 4050 mg/kg/81W- intermittentTumorigenic: Neoplastic by RTECS criteria

Gastrointestinal: Tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
HPV392 -,-,2004
oral/rat lowest published toxic dose: 3700 mg/kg/81W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors
ZEKBAI 74,241,1970
skin/mouse lowest published toxic dose: 78144 mg/kg/88W- intermittentTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
HPV392 -,-,2004
subcutaneous/rat lowest published toxic dose: 800 mg/kg/32W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Tumorigenic: Tumors at site of application
ZEKBAI 74,241,1970
subcutaneous/rat lowest published toxic dose: 2450 mg/kg/49W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Skin and Appendages: Tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
HPV392 -,-,2004

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Hamster, lung fibroblast Inhibitor Concentration Low: 0.1 mmol/L/48HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TXCYAC 275,58,2010
In Vitro/Hamster, lung fibroblast Inhibitor Concentration Low: 5 mmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TXCYAC 275,58,2010
In Vitro/Hamster, lung fibroblast Inhibitor Concentration (50 percent kill): 3.99 mmol/L/48HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TXCYAC 275,58,2010
In Vitro/Hamster, lung fibroblast Inhibitor Concentration (50 percent kill): 8.06 mmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TXCYAC 275,58,2010
In Vitro/Hamster, lung fibroblast Inhibitor Concentration Low: 0.3 mmol/L/48HIn Vitro Toxicity Studies: Other assaysTXCYAC 275,58,2010
In Vitro/Hamster, lung fibroblast Inhibitor Concentration Low: 3 mmol/L/12HIn Vitro Toxicity Studies: Other assaysTXCYAC 275,58,2010
inhalation/rat Lowest published lethal concentration: 250 ppm/4HJIHTAB 31,343,1949
oral/mouse lethal dose (50 percent kill): 647 mg/kgNTIS** PB214-270
oral/rat lethal dose (50 percent kill): 880 mg/kgHPV392 -,-,2004
skin/rabbit lethal dose (50 percent kill): 706 mg/kgHPV392 -,-,2004
skin/rabbit lethal dose (50 percent kill): 600 µL/kgUCDS** 12/30/1971
subcutaneous/rat lethal dose (50 percent kill): 350 mg/kgHPV392 -,-,2004

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 4,277,1974
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 54,213,1992
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 71,1405,1999
International Agency for Research on Cancer (IARC)Cancer Review:Human Limited EvidenceIMSUDL 7,198,1987
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 54,213,1992
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 71,1405,1999
International Agency for Research on Cancer (IARC)Cancer Review:Group 2AIMEMDT 71,1405,1999
TOXICOLOGY REVIEWMUREAV 543,201,2003
TOXICOLOGY REVIEWMUREAV 613,17,2006
TOXICOLOGY REVIEWMUTAEX 20,389,2005
TOXICOLOGY REVIEWFCTOD7 44,1699,2006
TOXICOLOGY REVIEWMUREAV 721,27,2011

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-AUSTRIATRK-TMW 0.03 ppm (0.2 mg/m3);KZW 0.12 ppm (0.8 mg/m3), skin, 2007
Occupational Exposure Limit-HUNGARYceiling concentration 0.2 mg/m3, Carcinogen, SEP2000
Occupational Exposure Limit-JAPAN2A carc, MAY2012
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 0.05 ppm (0.32 mg/m3), skin, JAN2002
Occupational Exposure Limit-SWEDENGroup B Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 0.03 ppm (0.2 mg/m3), carc 2, JAN2011
Occupational Exposure Limit-UNITED KINGDOMtime-weighted average 0.05 ppm (0.32 mg/m3), skin, OCT2007

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code 84284; Number of Industries 4; Total Number of Facilities 86; Number of Occupations 3; Total Number of Employees Exposed 2260; Total Number of Female Employees Exposed 165
National Occupational Hazard Survey 1974Hazard Code 84284; Number of Industries 4; Total Number of Facilities 116; Number of Occupations 11; Total Number of Employees Exposed 1646

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: Cytogenetics-male germ cell; Mouse specific locus
EPA GENETOX PROGRAM 1988, Negative: D melanogaster-whole sex chrom. loss
EPA GENETOX PROGRAM 1988, Negative: Rodent dominant lethal
EPA GENETOX PROGRAM 1988, Positive: Aspergillus-forward mutation; Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive: D melanogaster-reciprocal translocation
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: Host-mediated assay; N crassa-reversion
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion; S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion; S pombe-reversion
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULEFEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NTP 11th Report on Carcinogens,2004:Reasonably anticipated to be a human carcinogen
On EPA IRIS database
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