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Registry of Toxic Effects of Chemical Substances (RTECS)

p-Phenylenediamine, 2-nitro-

RTECS #

ST3000000

CAS #

5307-14-2

Updated

October 2011

Molecular Weight

153.16

Molecular Formula

C6H7N3O2

Synonyms

1,4-Diamino-2-nitrobenzene
1,4-Diaminonitrobenzol (German)
2-Nitro-1,4-benzenediamine
2-Nitro-1,4-diaminobenzene
2-Nitro-1,4-phenylenediamine
2-Nitro-4-aminoaniline
2-Nitro-p-phenylenediamine
4-Amino-2-nitroaniline
C.I. 76070
C.I. Oxidation Base 22
Durafur Brown 2R
Dye GS
Fouramine 2R
Fourrine 36
Fourrine Brown 2R
NCI-C02222
Nitro-p-phenylenediamine
o-Nitro-p-phenylenediamine
Oxidation Base 22
Ursol Brown RR
Zoba Brown RR

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
skin /woman 1%CODEDG 60,118,2009

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
body fluid assaySalmonella typhimurium/rat200 µg/kgMUREAV 66,241,1979
Cytogenetic Analysislymphocyte/human50 mg/L/24HNATUAS 255,506,1975
Cytogenetic Analysisovary/hamster123 mg/LEMMUEG 27,67,1996
Cytogenetic Analysisfibroblast/hamster8 mg/L/48HMUREAV 48,337,1977
Cytogenetic Analysisother cell types/hamster10 µmol/L/24HNATUAS 260,368,1976
Cytogenetic Analysisother cell types/hamster10 µmol/LNATUAS 260,368,1976
Cytogenetic Analysislung/hamster10 mg/LATSUDG 4,41,1980
DNA Damagelymphocyte/mouse5970 µmol/LMUREAV 203,155,1988
DNA repair/Escherichia coli4170 µmol/LMUREAV 272,145,1992
host-mediated assayEscherichia coli/mouse160 mg/kgMUREAV 272,161,1992
micronucleus testintraperitoneal/mouse400 mg/kg/24H-continuousCYTOAN 57,149,1992
morphological transformembryo/mouse10 µmol/L/24HNATUAS 260,368,1976
morphological transformother cell types/mouse10 µmol/LNATUAS 260,368,1976
morphological transformembryo/hamster500 µg/LNCIMAV 58,243,1981
mutation in mammalian somatic cellslymphocyte/mouse25 mg/LJEPTDQ 1(1),87,1977
mutation in microorganismslymphocyte/mouse5 mg/L (+enzymatic activation step)EMMUEG 12(Suppl 13),103,1988
mutation in microorganisms/Salmonella typhimurium5 µg/plate (+enzymatic activation step)NATUAS 255,506,1975
mutation in microorganisms/Salmonella typhimurium3300 ng/plate (-enzymatic activation step)ENMUDM 7(Suppl 5),1,1985
mutation in microorganisms/Escherichia coli333 µg/plate (-enzymatic activation step)ENMUDM 7(Suppl 5),1,1985
mutation in microorganisms/Escherichia coli100 µg/plate (+enzymatic activation step)ENMUDM 7(Suppl 5),1,1985
sister chromatid exchangeovary/hamster10 mg/LMUREAV 56,207,1977
sister chromatid exchangeoral/hamster125 mg/kgBLFSBY 29B,613,1984
specific locus testovary/hamster100 mg/L/9DEMMUEG 16,260,1990
Unscheduled DNA SynthesisHeLa cell/human100 nmol/LCNREA8 38,2621,1978
Unscheduled DNA Synthesisliver/rat100 mg/LMUREAV 97,359,1982

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
subcutaneous/mouse 1600 mg/kg (6-15D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)

Reproductive: Specific developmental abnormalities: Craniofacial (including nose and tongue)

Reproductive: Specific developmental abnormalities: Cardiovascular (circulatory) system
TJADAB 19,37A,1979
subcutaneous/mouse 1600 mg/kg (6-15D pregnant)Reproductive: Specific developmental abnormalities: Craniofacial (including nose and tongue)

Reproductive: Specific developmental abnormalities: Musculoskeletal system
TJADAB 24,253,1981
subcutaneous/mouse 2240 mg/kg (6-15D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)

Reproductive: Specific developmental abnormalities: Eye, ear
TJADAB 24,253,1981
subcutaneous/mouse 1280 mg/kg (6-15D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)TJADAB 24,253,1981

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 288 gm/kg/78W- continuousTumorigenic: Neoplastic by RTECS criteria

Liver: Tumors
NCITR* NCI-TR-169,1979
oral/mouse toxic dose: 144 gm/kg/78W- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Liver: Tumors
NCITR* NCI-TR-169,1979

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse lethal dose (50 percent kill): 214 mg/kgGPOLA4 26,109,1985
intraperitoneal/rat lethal dose (50 percent kill): 348 mg/kgJTEHD6 2,657,1977
oral/rat lethal dose (50 percent kill): 2100 mg/kgKidney, Ureter, and Bladder: Other changes in urine compositionJSCCA5 23,259,1972

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMEMDT 57,185,1993
International Agency for Research on Cancer (IARC)Cancer Review:Animal Inadequate EvidenceIMEMDT 16,73,1978
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 16,73,1978
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 57,185,1993
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMEMDT 57,185,1993
TOXICOLOGY REVIEWJACTDZ 4(3),161,1985
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWMUREAV 590,1,2005
TOXICOLOGY REVIEWTXAPA9 243,239,2010
TOXICOLOGY REVIEWTXAPA9 243,239,2010
TOXICOLOGY REVIEWMUREAV 705,184,2010

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code X1644; Number of Industries 1; Total Number of Facilities 3160; Number of Occupations 1; Total Number of Employees Exposed 29422; Total Number of Female Employees Exposed 23531

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: Mammalian micronucleus; In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Negative: Rodent dominant lethal
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Positive/limited: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: SHE-clonal assay; Cell transform.-C3H/10T1/2
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, PROPOSED RULEFEREAC 47,27009,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);clear evidence:mouseNCITR* NCI-TR-169,1979
NCI Carcinogenesis Bioassay (feed);no evidence:ratNCITR* NCI-TR-169,1979
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