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Registry of Toxic Effects of Chemical Substances (RTECS)

o-Phenylenediamine, 4-nitro-

RTECS #

ST2975000

CAS #

99-56-9

Updated

April 2013

Molecular Weight

153.16

Molecular Formula

C6H7N3O2

Synonyms

1,2-Diamino-4-nitrobenzene
2-Amino-4-nitroaniline
3,4-Diaminonitrobenzene
4NDB
4-Nitro-1,2-benzenediamine
4-Nitro-1,2-diaminobenzene
4-Nitro-1,2-fenylendiamin (Czech)
4-Nitro-1,2-phenylenediamine
4-Nitro-o-phenylenediamine
4-Nitro-o-phenylene-diamine
4-NO
4-Nop
4-Nopd
4-N-o-Pda
C.I. 76020
NCI-C03941
NOPD
p-Nitro-o-phenylenediamine

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisovary/hamster138 mg/LEMMUEG 27,67,1996
Cytogenetic Analysisfibroblast/hamster25 mg/LMUREAV 40,47,1976
Cytogenetic Analysisother cell types/hamster10 µmol/L/24HNATUAS 260,368,1976
Cytogenetic Analysisother cell types/hamster10 µmol/LNATUAS 260,368,1976
Cytogenetic Analysislung/hamster40 mg/LGMCRDC 27,95,1981
DNA Damagelymphocyte/mouse104 µmol/LMUREAV 203,155,1988
DNA Damage/Salmonella typhimurium30 µmol/L/2HEMMUEG 46,126,2005
DNA Damagemammary gland/human7.6 mg/L/24H (-enzymatic activation step)MUREAV 588,47,2005
DNA Damageliver/mouse2 mmol/L/24HMUTAEX 25,561,2010
DNA Damageliver/mouse2 mmol/L/48HMUTAEX 25,561,2010
DNA repair/Escherichia coli10 mg/LCRNGDP 2,189,1981
Dominant Lethal Testintraperitoneal/mouse3000 mg/kg/10W- intermittentSheCW# 25MAR1977
gene conversion and mitotic recombination/Saccharomyes cerevisiae500 mg/LMUREAV 78,243,1980
micronucleus testintraperitoneal/mouse1 gm/kgEMMUEG 14,207,1989
morphological transformembryo/mouse10 µmol/L/24HNATUAS 260,368,1976
morphological transformother cell types/mouse10 µmol/LNATUAS 260,368,1976
morphological transformembryo/rat80 µg/plateJJATDK 1,190,1981
morphological transformembryo/hamster500 µg/LNCIMAV 58,243,1981
mutation in mammalian somatic cellslymphocyte/mouse50 mg/LJEPTDQ 1(1),87,1977
mutation in microorganisms/Salmonella typhimurium10 µg/plate (-enzymatic activation step)JAPTO* 20,491,2000
mutation in microorganisms/Salmonella typhimurium0.01 mg/plate/20M (-enzymatic activation step)EMMUEG 36,13,2000
mutation in microorganisms/Salmonella typhimurium2 µL/plate (-enzymatic activation step)VHTODE 42,77,2000
mutation in microorganisms/Salmonella typhimurium0.02 mg/plate/45M (-enzymatic activation step)TCMUD8 22,285,2002
mutation in microorganisms/Escherichia coli0.00025 mg/plate/90M (-enzymatic activation step)MUREAV 535,55,2003
mutation in microorganisms/Salmonella typhimurium108000 ng/plate/72H (+enzymatic activation step)MUREAV 542,49,2003
mutation in microorganisms/Salmonella typhimurium0.01 µmol/plate/72H (-enzymatic activation step)MUREAV 558,93,2004
mutation in microorganisms/Salmonella typhimurium0.5 µg/plate/48H (+enzymatic activation step)EMMUEG 44,387,2004
mutation in microorganisms/Salmonella typhimurium5 µg/plate/72H (-enzymatic activation step)MUREAV 585,105,2005
mutation in microorganisms/Salmonella typhimurium2 gm/L (+enzymatic activation step)DCTODJ 27,157,2004
mutation in microorganisms/Salmonella typhimurium2 gm/L (-enzymatic activation step)DCTODJ 27,157,2004
mutation in microorganisms/Salmonella typhimurium8 µg/plate/30MDCTODJ 27,157,2004
mutation in microorganisms/Salmonella typhimurium0.4 µmol/L/16HEMMUEG 46,126,2005
mutation in microorganisms/Salmonella typhimurium0.4 µmol/L/48HEMMUEG 46,126,2005
mutation in microorganisms/Salmonella typhimurium30 µmol/L/2HEMMUEG 46,126,2005
mutation in microorganisms/Aspergillus nidulans200 mg/L (-enzymatic activation step)MUREAV 97,293,1982
mutation in microorganismslymphocyte/mouse13100 µg/L (+enzymatic activation step)EMMUEG 12(Suppl 13),37,1988
mutation in microorganisms/Salmonella typhimurium1 µg/plate (+/-enzymatic activation step)ENMUDM 8(Suppl 7),1,1986
mutation in microorganisms/Bacillus subtilis50 mg/L (-enzymatic activation step)MUREAV 95,191,1982
mutation in microorganisms/Salmonella typhimurium200000 µg/L/48H (-enzymatic activation step)FCTOD7 48,320,2010
mutation in microorganisms/Salmonella typhimurium10 µg/plate/48H (-enzymatic activation step)MUREAV 699,47,2010
mutation in microorganisms/Salmonella typhimurium10 µg/plate/48HFCTOD7 49,1753,2011
mutation in microorganisms/Salmonella typhimurium0.02 mg/plateFCTOD7 49,2676,2011
mutation in microorganisms/Other microorganisms80 mg/L/4HMUREAV 611,17,2006
mutation in microorganisms/Salmonella typhimurium10 µg/plate/30M (-enzymatic activation step)CPBTAL 55,1635,2007
other mutation test systems/Salmonella typhimurium5 mg/LMUREAV 192,239,1987
other mutation test systemsoral/mouse250 mg/kgMUREAV 343,157,1995
phage inhibition capacity/Escherichia coli400 µg/plateMGBUA3 41,3,1976
sex chromosome loss and nondisjunctionparenteral/Drosophila melanogaster5 mmol/LMUREAV 56,31,1977
sex chromosome loss and nondisjunctionoral/Drosophila melanogaster1200 µmol/L/3DMUREAV 48,181,1977
sister chromatid exchangeovary/hamster25 mg/LMUREAV 56,207,1977
specific locus testoral/Drosophila melanogaster2 mmol/LMUREAV 341,161,1995
specific locus test/Escherichia coli0.25 µg/plate/12H (-enzymatic activation step)MUREAV 535,55,2003
Unscheduled DNA SynthesisHeLa cell/human1 mmol/LCNREA8 38,2621,1978
Unscheduled DNA Synthesisliver/rat500 µg/LEMMUEG 26,147,1995

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat 3 gm/kg (30D male)Reproductive: Effects on embryo or fetus: Fetal deathSheCW# 25MAR1977
subcutaneous/mouse 2560 mg/kg (6-15D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)

Reproductive: Specific developmental abnormalities: Craniofacial (including nose and tongue)

Reproductive: Specific developmental abnormalities: Cardiovascular (circulatory) system
TJADAB 19,37A,1979
subcutaneous/mouse 7680 mg/kg (6-15D pregnant)Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth)TJADAB 24,253,1981

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat lethal dose (50 percent kill): >1600 mg/kgJTEHD6 2,657,1977
oral/mouse lethal dose (50 percent kill): 681 mg/kgNCILB* NCI-E-C-72-3252,1973
oral/rat lethal dose (50 percent kill): 681 mg/kgNCILB* NCI-E-C-72-3252,1973

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Inadequate EvidenceIMEMDT 16,63,1978
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 16,63,1978
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMSUDL 7,56,1987
TOXICOLOGY REVIEWJACTDZ 4(3),161,1985
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWMUTAEX 23,271,2008
TOXICOLOGY REVIEWTXAPA9 243,239,2010
TOXICOLOGY REVIEWMUREAV 705,184,2010

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code A1601; Number of Industries 1; Total Number of Facilities 2065; Number of Occupations 1; Total Number of Employees Exposed 17652; Total Number of Female Employees Exposed 11624

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Inconclusive: Rodent dominant lethal; Mammalian micronucleus
EPA GENETOX PROGRAM 1988, Negative: Carcinogenicity-mouse/rat; N crassa-aneuploidy
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; S cerevisiae-reversion
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Positive: Body fluid assay; SHE-clonal assay
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-C3H/10T1/2
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULEFEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);no evidence:mouse,ratNCITR* NCI-TR-180,1979
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