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Registry of Toxic Effects of Chemical Substances (RTECS)

Phenol, 2,4-dinitro-

RTECS #

SL2800000

CAS #

51-28-5

Updated

April 2012

Molecular Weight

184.12

Molecular Formula

C6H4N2O5

Synonyms

1-Hydroxy-2,4-dinitrobenzene
2,4-Dinitrofenol (Dutch)
2,4-Dinitrophenol
2,4-Dnp
Aldifen
alpha-Dinitrophenol
Chemox PE
Dinitrofenolo (Italian)
Dinofan
Fenoxyl carbon N
Maroxol-50
Nitro kleenup
NSC 1532
Phenol, alpha-dinitro-
RCRA waste number P048
Solfo Black 2B Supra
Solfo Black B
Solfo Black BB
Solfo Black G
Solfo Black SB
Tertrosulphur Black PB
Tertrosulphur PBR

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
skin /rabbit 300 mg/4W- intermittent mildJIHTAB 30,10,1948

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisintraperitoneal/mouse10 gm/kgIJMRAQ 59,1442,1971
DNA Damageliver/human0.316 mmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human0.1 mmol/l/48HMUREAV 696,21,2010
DNA Damageother cell types/mouse50 mg/L/3H (-enzymatic activation step)MUREAV 541,9,2003
DNA Damageliver/rat100 µmol/LMUREAV 368,59,1996
DNA inhibitionlung/hamster7 mmol/LMUREAV 243,213,1990
micronucleus testother cell types/mouse2 mmol/l/4HMUTAEX 22,275,2007
mutation in microorganisms/Escherichia coli200 ppm/3H (-enzymatic activation step)AMNTA4 85,119,1951
mutation in microorganisms/Saccharomyes cerevisiae31.6 µmol/L/6HMUREAV 676,113,2009
other mutation test systemsother cell types/other fish100 µmol/LAEEXAH (3),279,1972
specific locus testother cell types/mouse2 mmol/l/4HMUTAEX 22,275,2007

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse 40800 µg/kg (10-12D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)FCTXAV 11,31,1973
oral/rat 2040 mg/kg (8D pre-21D after birth)Reproductive: Effects on newborn: Stillbirth

Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4)
PSEBAA 32,678,1935

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Hamster, kidney Inhibitor Concentration (20 percent kill): 179 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (20 percent kill): 109 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (20 percent kill): 216 µmol/L/24HIn Vitro Toxicity Studies: Cell protein synthesisTIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (50 percent kill): 370 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (50 percent kill): 311 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (50 percent kill): 505 µmol/L/24HIn Vitro Toxicity Studies: Cell protein synthesisTIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (80 percent kill): 898 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 7,149,1993
In Vitro/Hamster, kidney Inhibitor Concentration (80 percent kill): 606 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 7,149,1993
In Vitro/Hamster, lung fibroblast Inhibitor Concentration (50 percent kill): 15 mg/L/72HIn Vitro Toxicity Studies: Cell protein synthesisTIVIEQ 4,175,1990
In Vitro/Mouse, fibroblast Inhibitor Concentration (10 percent kill): 109 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 2,1,1988
In Vitro/Mouse, fibroblast Inhibitor Concentration (50 percent kill): 1358 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 2,1,1988
In Vitro/Mouse, fibroblast Inhibitor Concentration (10 percent kill): 109 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 2,1,1988
In Vitro/Mouse, fibroblast Inhibitor Concentration (50 percent kill): 1630 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 2,1,1988
inhalation/Dog Lowest published lethal concentration: 300 mg/m3/30M85GMAT -,62,1982
intramuscular/pigeon lethal dose (50 percent kill): 6500 µg/kgNutritional and Gross Metabolic: Body temperature increaseJPETAB 53,58,1935
intraperitoneal/guinea pig lethal dose (50 percent kill): 28 mg/kgARZNAD 8,381,1958
intraperitoneal/mouse lethal dose (50 percent kill): 26 mg/kgBCPCA6 18,1389,1969
intraperitoneal/rat lethal dose (50 percent kill): 20 mg/kgJPPMAB 17,814,1965
intravenous/Dog lowest published lethal dose: 15 mg/kgJPETAB 49,187,1933
intravenous/mouse lethal dose (50 percent kill): 56 mg/kgBehavioral: Altered sleep time (including change in righting reflex)

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Respiratory stimulation
TXAPA9 6,232,1964
intravenous/rat lethal dose (50 percent kill): 72 mg/kgBehavioral: Altered sleep time (including change in righting reflex)

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Respiratory stimulation
TXAPA9 6,232,1964
oral/cat lethal dose (50 percent kill): 75 mg/kg85GMAT -,62,1982
oral/Dog lowest published lethal dose: 30 mg/kgJPETAB 49,187,1933
oral/guinea pig lethal dose (50 percent kill): 81 mg/kgBehavioral: Change in motor activity (specific assay)

Behavioral: Ataxia

Behavioral: Tetany
FATOAO 28,493,1965
oral/human lowest published lethal dose: 36 mg/kgBehavioral: Coma

Cardiac: Change in rate

Nutritional and Gross Metabolic: Body temperature increase
JAMAAP 101,1333,1933
oral/mouse lethal dose (50 percent kill): 45 mg/kgBehavioral: Change in motor activity (specific assay)

Behavioral: Ataxia

Behavioral: Tetany
FATOAO 28,493,1965
oral/mouse lethal dose (50 percent kill): 72 mg/kgHBPTO* 2,1229,2001
oral/rabbit lethal dose (50 percent kill): 30 mg/kgBehavioral: Change in motor activity (specific assay)

Behavioral: Ataxia

Behavioral: Tetany
FATOAO 28,493,1965
oral/rat lowest published toxic dose: 30 mg/kgBehavioral: Change in motor activity (specific assay)

Gastrointestinal: Changes in structure or function of salivary glands
JTSCDR 26,299,2001
oral/rat lethal dose (50 percent kill): 30 mg/kgHBPTO* 2,1229,2001
oral/wild bird lethal dose (50 percent kill): 13 mg/kgTXAPA9 21,315,1972
skin/guinea pig lowest published lethal dose: 700 mg/kgJIHTAB 30,10,1948
subcutaneous/Dog lowest published lethal dose: 20 mg/kgJPETAB 49,187,1933
subcutaneous/guinea pig lowest published lethal dose: 25 mg/kgNutritional and Gross Metabolic: Body temperature increaseAEPPAE 192,331,1939
subcutaneous/mouse lethal dose (50 percent kill): 58 mg/kgBehavioral: Altered sleep time (including change in righting reflex)

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Respiratory stimulation
TXAPA9 6,232,1964
subcutaneous/rabbit lowest published lethal dose: 20 mg/kgJPETAB 49,187,1933
subcutaneous/rat lethal dose (50 percent kill): 25 mg/kgJPETAB 49,187,1933
unreported route/mammal (species unspecified) lethal dose (50 percent kill): 40 mg/kg30ZDA9 -,97,1971

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/chicken lowest published toxic dose: 10230 mg/kg/28D- continuousEye: Other eye effects

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** OTS0545267
oral/rabbit lowest published toxic dose: 126 µg/kg/30W- intermittentBlood: Other changesGISAAA 31(6),3,1966
oral/rat lowest published toxic dose: 6510 µg/kg/30W- intermittentBehavioral: Alteration of classical conditioningGISAAA 31(6),3,1966
oral/rat lowest published toxic dose: 540 mg/kg/18D- intermittentBehavioral: Somnolence (general depressed activity)

Behavioral: Convulsions or effect on seizure threshold

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JTSCDR 26,299,2001
oral/rat lowest published toxic dose: 140 mg/kg/7D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainJTSCDR 26,299,2001
oral/rat lowest published toxic dose: 200 mg/kg/10D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainJTSCDR 26,299,2001
oral/rat lowest published toxic dose: 180 mg/kg/18D- intermittentRelated to Chronic Data: Changes in testicular weightJTSCDR 26,299,2001
oral/rat lowest published toxic dose: 360 mg/kg/18D- intermittentBrain and Coverings: Other degenerative changesJTSCDR 26,299,2001
oral/rat lowest published toxic dose: 360 mg/kg/18D- intermittentBlood: Changes in erythrocyte (RBC) countJTSCDR 26,299,2001
oral/rat lowest published toxic dose: 2240 mg/kg/28D- intermittentBehavioral: Convulsions or effect on seizure threshold

Behavioral: Rigidity (includes catalepsy)

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JTSCDR 26,299,2001
oral/rat lowest published toxic dose: 360 mg/kg/18D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainTOXID9 66,152,2002
oral/rat lowest published toxic dose: 540 mg/kg/18D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldTOXID9 66,152,2002
oral/rat lowest published toxic dose: 2240 mg/kg/28D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldTOXID9 66,152,2002

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWCRTXB2 1(1),93,1971
TOXICOLOGY REVIEWEMMUEG 43,36,2004
TOXICOLOGY REVIEWENTOX* -,59,2005
TOXICOLOGY REVIEWREPTED 26,185,2008
TOXICOLOGY REVIEWMUREAV 672,10,2009
TOXICOLOGY REVIEWMUREAV 672,17,2009
TOXICOLOGY REVIEWTPHSDY 29,361,2008
TOXICOLOGY REVIEWMUTAEX 22,161,2007
TOXICOLOGY REVIEWNBREV* 5,1,1981
TOXICOLOGY REVIEWMUREAV 741,32,2012

Standards and Regulations

OrganizationStandardReference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATIONFEREAC 54,7740,1989
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDESCancelledRBREV* -,175,1998
Occupational Exposure Limit-RUSSIAtime-weighted average 0.05 mg/m3, short term exposure limit 0.2 mg/m3, Skin, JUN2003

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code 27110; Number of Industries 1; Total Number of Facilities 7; Number of Occupations 3; Total Number of Employees Exposed 130; Total Number of Female Employees Exposed 14
National Occupational Hazard Survey 1974Hazard Code 27110; Number of Industries 1; Total Number of Facilities 19; Number of Occupations 1; Total Number of Employees Exposed 38

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/264339)
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
On EPA IRIS database
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