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Nitrous acid, isobutyl ester

RTECS #: RA0805000

CAS #: 542-56-3

UPDATE: May 2009 MW: 103.14 MF: C4H9NO2


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. MUTATION DATA:
  3. TUMORIGENIC DATA:
  4. ACUTE TOXICITY DATA:
  5. OTHER MULTIPLE DOSE DATA:
  6. REVIEWS:
  7. STANDARDS AND REGULATIONS:
  8. NIOSH DOCUMENTATION AND SURVEILLANCE:
  9. STATUS IN FEDERAL AGENCIES:
  10. REFERENCES:

SYNONYMS:

  1. Blackjack
  2. Isobutyl nitrite
  1. NCI - C61052
  2. Nitrous acid, 2 - methylpropyl ester (9CI)


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE 		DOSE REFERENCE
DNA damage human lung 3.9 µmol/L/20 hour MUREAV 605,94,2006
DNA damage rat lung 7.8 µmol/L/20 hour MUREAV 605,94,2006
DNA damage oral
rat 		606 mg/kg MUREAV 605,94,2006
mutation in microorganisms Salmonella typhimurium 1 mg/plate (+enzymatic activation step) BSIBAC 56,816,1980
mutation in microorganisms Salmonella typhimurium 333 µg/plate (-enzymatic activation step) NTPTB* APR1982
mutation in microorganisms Salmonella typhimurium 28 mg/L/2 hour (-enzymatic activation step) MUREAV 578,210,2005
mutation in microorganisms Salmonella typhimurium 500 mg/L/2 hour (+enzymatic activation step) MUREAV 578,210,2005
mutation in mammalian somatic cells mouse lymphocyte 75,900 nmol/L EMMUEG 14,115,1989


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
mouse 		lowest published toxic concentration: 150 ppm/6 hour/2 year- intermittent Tumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors 		NTPTR* NTP-TR-448,1996
inhalation
mouse 		lowest published toxic concentration: 9,270 mg/kg/103 week- intermittent Tumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors 		NTIS** PB97-120232
inhalation
rat 		lowest published toxic concentration: 150 ppm/6 hour/2 year- intermittent Tumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors 		NTPTR* NTP-TR-448,1996
inhalation
rat 		lowest published toxic concentration: 4,635 mg/kg/103 week- intermittent Tumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors 		NTIS** PB97-120232
inhalation
rat 		lowest published toxic concentration: 3,862.5 mg/kg/103 week- intermittent Tumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors 		NTIS** PB97-120232


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
mouse 		lethal concentration (50 percent kill): 1,033 ppm/1 hour Behavioral: Change in motor activity (specific assay)

Lung, Thorax, or Respiration: Cyanosis

Kidney, Ureter, and Bladder: Urine volume increased 		FAATDF 1,448,1981
inhalation
mouse 		lowest published toxic concentration: 900 ppm/45 minute Endocrine: Other changes TOLED5 116,151,2000
inhalation
rat 		lethal concentration (50 percent kill): 777 ppm/4 hour Behavioral: Altered sleep time (including change in righting reflex)

Behavioral: Excitement

Lung, Thorax, or Respiration: Cyanosis 		FAATDF 8,101,1987
intraperitoneal
mouse 		lethal dose (50 percent kill): 184 mg/kg Liver: Other changes RCOCB8 26,75,1979
oral
man 		lowest published toxic dose: 120 mg/kg Blood: Methemoglobinemia- Carboxhemoglobinemia

Vascular: BP lowering not characterized in autonomic section

Cardiac: Change in rate 		AIMEAS 92,637,1980
oral
mouse 		lethal dose (50 percent kill): 205 mg/kg Liver: Other changes

Blood: Methemoglobinemia- Carboxhemoglobinemia 		RCSADO 3,233,1982
oral
rat 		lethal dose (50 percent kill): 410 mg/kg Behavioral: General anesthetic FEPRA7 41,1583,1982


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
mouse 		lowest published toxic concentration: 400 ppm/7 hour/60 day- intermittent Liver: Changes in liver weight

Kidney, Ureter, and Bladder: Changes in bladder weight

Endocrine: Changes in spleen weight 		JJATDK 5,134,1985
inhalation
mouse 		lowest published toxic concentration: 800 ppm/6 hour/2 week- intermittent Lung, Thorax, or Respiration: Changes in lung weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field 		FAATDF 19,169,1992
inhalation
mouse 		lowest published toxic concentration: 300 ppm/6 hour/14 week- intermittent Lung, Thorax, or Respiration: Structural or functional change in trachea or bronchi

Blood: Changes in erythrocyte (RBC) count

Blood: Changes in leukocyte (WBC) count 		FAATDF 19,169,1992
inhalation
mouse 		lowest published toxic concentration: 300 ppm/6.5 hour/18 week- intermittent Liver: Changes in liver weight

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in leukocyte (WBC) count 		JTEHD6 15,823,1985
inhalation
mouse 		lowest published toxic concentration: 900 ppm/5 day- intermittent Immunological Including Allergic: Decreased immune response

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation 		TOLED5 152,47,2004
inhalation
rat 		lowest published toxic concentration: 600 ppm/6 hour/2 week- intermittent Lung, Thorax, or Respiration: Other changes

Liver: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field 		FAATDF 19,169,1992
inhalation
rat 		lowest published toxic concentration: 150 ppm/6 hour/13 week- intermittent Olfaction: Other olfaction effects

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in erythrocyte (RBC) count 		FAATDF 19,169,1992


REVIEWS:

ORGANIZATION STANDARD
REFERENCE
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value ceiling concentration 1 ppm (inhalable, vapor) DTLVS* TLV/BEI,2007
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value Confirmed animal carcinogen DTLVS* TLV/BEI,2007
TOXICOLOGY REVIEW
ENTOX* -,231,2005
TOXICOLOGY REVIEW
MUREAV 584,1,2005
TOXICOLOGY REVIEW
MUREAV 659,202,2008
TOXICOLOGY REVIEW
MUREAV 659,202,2008
TOXICOLOGY REVIEW
HUTOX* -,577,1996


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE
Occupational Exposure Limit - BELGIUM short term exposure limit 1 ppm (4.3 mg/m3) (vapor, aer), Carcinogen, MAR2002


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: X4720;
Number of Industries 1;
Total Number of Facilities 13;
Number of Occupations 4;
Total Number of Employees Exposed 290;
Total Number of Female Employees Exposed 13


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE
EPA TSCA Section 8(b) CHEMICAL INVENTORY
Used as an intermediate in the syntheses of aliphatic nitrites
NCI Carcinogenesis Studies (inhal);clear evidence:rat
NTP Carcinogenesis studies (inhalation);some evidence:mouse NTPTR* NTP-TR-448,1996
NCI Carcinogenesis Studies (inhal);equivocal evidence:mouse


REFERENCES:

CODEN
REFERENCE
AIMEAS Annals of Internal Medicine. (American College of Physicians, 4200 Pine St., Philadelphia, PA 19104) V.1- 1927-
BSIBAC Bolletino della Societe Italiana di Biologia Sperimentale. (Casa Editrice Idelson, Via A. de Gasperi, 55, 80133 Naples, Italy) V.2- 1927-
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987-
ENTOX* Encyclopedia of Toxicology: Reference Book, Elsevier, 2005
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1-40, 1981-97. For publisher information, see TOSCF2
FEPRA7 Federation Proceedings, Federation of American Societies for Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
HUTOX*
JJATDK JAT, Journal of Applied Toxicology. (John Wiley & Sons Ltd., Baffins Lane, Chichester, W. Sussex PO19 1UD, UK) V.1- 1981-
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964-
NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information.
NTPTB* NTP Technical Bulletin. (National Toxicology Program, Landow Bldg. 3A-06, 7910 Woodmont Ave., Bethesda, MD 20205)
NTPTR* National Toxicology Program Technical Report Series. (Research Triangle Park, NC 27709) No.206-
RCOCB8 Research Communications in Chemical Pathology and Pharmacology. (PJD Pub. Ltd., P.O. Box 966, Westbury, NY 11590) V.1- 1970-
RCSADO Research Communications in Substances Abuse. (PJD Pub. Ltd., P.O.Box 966, Westbury, NY 11590) V.1- 1980-
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1977-

Used as an intermediate in the syntheses of aliphatic nitrites

RTECS Compound Description:
   Tumorigen
   Mutagen
   Human Data

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