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Registry of Toxic Effects of Chemical Substances (RTECS)

Hydrazine, 1,2-dimethyl-

RTECS #

MV2625000

CAS #

540-73-8

Updated

September 201

Molecular Weight

60.12

Molecular Formula

C2H8N2

Synonyms

1,2-Dimethylhydrazin (German)
1,2-Dimethylhydrazine
1,2-Dimethyl-hydrazine
Hydrazomethane
N,N'-Dimethylhydrazine
RCRA waste number U099
SDMH
sym-Dimethylhydrazine
Symetryczna dwumetylohydrazyna (Polish)

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisoral/mouse12 mg/kgJJIND8 71,133,1983
Cytogenetic Analysissubcutaneous/mouse15 mg/kgVOONC* 43,623,1997
Cytogenetic Analysisintraperitoneal/rat120 mg/kg/6WEMMUEG 44,113,2004
Cytogenetic Analysissubcutaneous/rat160 mg/kg/4D/2W- intermittentEMMUEG 45,8,2005
DNA adductlung/hamster2 mmol/LMUREAV 173,157,1986
DNA adductlung/human1290 µmol/LCNREA8 37,2309,1977
DNA adductfibroblast/human20 µmolNTIS** AD-A092-249
DNA adductother cell types/rat100 µmol/LTCMUD8 1,3,1980
DNA Damageintraperitoneal/rat10200 µg/kgCNREA8 41,3967,1981
DNA Damageliver/rat300 µmol/LSinJF# 26OCT1982
DNA Damageparenteral/rat210 mg/kgPAACA3 24,82,1983
DNA Damageoral/rat20 mg/kgENMUDM 4,667,1982
DNA Damageoral/mouse5700 µg/kgMUREAV 54,39,1978
DNA Damagesubcutaneous/mouse56200 µg/kgMUREAV 54,39,1978
DNA Damagesubcutaneous/rat40 mg/kgEMMUEG 45,8,2005
DNA Damageintraperitoneal/rat120 mg/kg/6WEMMUEG 44,113,2004
DNA Damagesubcutaneous/rat160 mg/kg/2W- intermittentTCMUD8 22,183,2001
DNA Damagesubcutaneous/rat180 mg/kg/6W- intermittentBPBLEO 30,569,2007
DNA Damageintraperitoneal/mouse20 mg/kgMUTAEX 16,39,2001
DNA inhibitionoral/mouse200 mg/kgMUREAV 46,305,1977
DNA inhibitionintraperitoneal/mouse60 mg/kgJTEHD6 13,117,1984
DNA repair/Bacillus subtilis6 mg/discMUREAV 97,1,1982
DNA repair/Escherichia coli250 µg/discJJIND8 62,873,1979
gene conversion and mitotic recombination/Saccharomyes cerevisiae200 mmol/LNATWAY 54,251,1967
host-mediated assayEscherichia coli/mouse50 µmol/kgMUREAV 148,1,1985
host-mediated assayintraperitoneal/mouse30 mg/kgMUREAV 564,1,2004
host-mediated assayintraperitoneal/mouse100 mg/kg/10W- intermittentMUREAV 564,1,2004
micronucleus testoral/rat3320 µg/kgMUREAV 368,115,1996
micronucleus testoral/mouse720 µg/kgMUREAV 368,115,1996
micronucleus testintraperitoneal/mouse10 mg/kgEMMUEG 17,155,1991
morphological transformintraperitoneal/rat500 µmol/kgCALEDQ 20,207,1983
morphological transformoral/rat500 µmol/kgCALEDQ 20,207,1983
morphological transformfibroblast/human230 µmol/LCALEDQ 29,265,1985
morphological transformembryo/hamster100 µg/LIJCNAW 19,642,1977
morphological transformoral/mouse30 mg/kg/8D- intermittentCNREA8 49,1236,1989
mutation in mammalian somatic cellslung/hamster100 µmol/LPAACA3 24,93,1983
mutation in mammalian somatic cellssubcutaneous/rat100 mg/kg/5W- intermittentCRNGDP 22,315,2000
mutation in mammalian somatic cellslymphocyte/mouse5 mmol/L/24HNTIS** AD-A075-605
mutation in microorganisms/Salmonella typhimurium25 µg/plate (+enzymatic activation step)CALEDQ 12,287,1981
mutation in microorganisms/Bacillus subtilis25 µg/plate (-enzymatic activation step)CALEDQ 12,287,1981
mutation in microorganismslymphocyte/mouse10 µL/L (+enzymatic activation step)NTIS** AD-A041-973
other mutation test systemssubcutaneous/mouse560 mg/kg/28W- intermittentCNREA8 43,2545,1983
phage inhibition capacity/Escherichia coli9400 mg/LCNREA8 41,532,1981
sister chromatid exchangeovary/hamster130 µmol/LMUREAV 68,351,1979
sister chromatid exchangeintraperitoneal/mouse270 µmol/kgTCMUD8 9,219,1989
sister chromatid exchangeintraperitoneal/mouse20 mg/kgMUTAEX 2,441,1987
sperm morphologysubcutaneous/mouse15 mg/kgVOONC* 43,623,1997
Unscheduled DNA Synthesissubcutaneous/mouse560 mg/kg/28W- intermittentCNREA8 43,2545,1983
Unscheduled DNA Synthesisintraperitoneal/mouse200 mg/kgJTEHD6 13,117,1984
Unscheduled DNA Synthesislung/human100 µL/LNTIS** AD-A041-973

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat 100 mg/kg (6-15D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)JTEHD6 13,125,1984

Tumorigenic Data and References

Route/OrganismDoseEffectReference
implant/rabbit lowest published toxic dose: 2 mg/kgTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Tumorigenic: Tumors at site of application
CNREA8 35,2292,1975
intraperitoneal/mouse lowest published toxic dose: 25 mg/kg/24W- intermittentTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors
TXAPA9 72,313,1984
intraperitoneal/mouse lowest published toxic dose: 360 mg/kg/12W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Gastrointestinal: Colon tumors
FCTOD7 50,1405,2012
intraperitoneal/rat lowest published toxic dose: 260 mg/kg/13W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors
CALEDQ 19,1,1983
oral/mouse lowest published toxic dose: 12500 µg/kg/24W- intermittentTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors
TXAPA9 72,313,1984
oral/rat lowest published toxic dose: 160 mg/kg/2W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
CALEDQ 168,23,2001
oral/rat lowest published toxic dose: 120 mg/kg/4W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Eye: Other eye effects
AJCNAC 30,176,1977
oral/rat lowest published toxic dose: 1750 mg/kg/2W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Endocrine: Thyroid tumors
CALEDQ 168,23,2001
parenteral/rat lowest published toxic dose: 160 mg/kg/16W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
PAACA3 21,67,1980
rectal/mouse lowest published toxic dose: 360 mg/kg/18W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
FEPRA7 34,827,1975
subcutaneous/hamster lowest published toxic dose: 340 mg/kg/17W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Behavioral: Ataxia

Gastrointestinal: Hypermotility, diarrhea
EXMPA6 27,19,1977
subcutaneous/monkey lowest published toxic dose: 400 mg/kg/2Y- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
JJCREP 83,584,1992
subcutaneous/monkey lowest published toxic dose: 608 mg/kg/2Y- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Reproductive: Tumorigenic effects: Uterine tumors
JJCREP 83,584,1992
subcutaneous/mouse lowest published toxic dose: 200 mg/kg/10W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Gastrointestinal: Colon tumors
JJIND8 63,1081,1979
subcutaneous/mouse toxic dose: 300 mg/kg/20W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Reproductive: Tumorigenic effects: Uterine tumors
JNCIAM 52,999,1974
subcutaneous/mouse lowest published toxic dose: 160 mg/kg/20W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Reproductive: Other reproductive system tumors
VOONC* 43,321,1997
subcutaneous/rat lowest published toxic dose: 160 mg/kg/2W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors
TCMUD8 22,183,2001
subcutaneous/rat lowest published toxic dose: 80 mg/kg/3W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors

Liver: Tumors
JTSCDR 27,57,2002
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
PHMREP 47,133,2003
subcutaneous/rat lowest published toxic dose: 160 mg/kg/2W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
TXAPA9 194,132,2004
subcutaneous/rat lowest published toxic dose: 80 mg/kg/4W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
CALEDQ 202,11,2003
subcutaneous/rat lowest published toxic dose: 260 mg/kg/21D- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Skin and Appendages: Tumors
FCTOD7 44,271,2006
subcutaneous/rat lowest published toxic dose: 180 mg/kg/6W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
BPBLEO K61
subcutaneous/rat lowest published toxic dose: 360 mg/kg/18W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
FCTOD7 48,390,2010
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
FCTOD7 47,2594,2009
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15WTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
FCTOD7 49,887,2011
subcutaneous/rat toxic dose: 120 mg/kg/12W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
NASDA6 32,270,1981
subcutaneous/rat toxic dose: 20 mg/kgTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Tumors

Gastrointestinal: Colon tumors
GANNA2 74,493,1983
subcutaneous/rat toxic dose: 200 mg/kg/10W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Kidney, Ureter, and Bladder: Kidney tumors
CRNGDP 3,1097,1982
subcutaneous/rat lowest published toxic dose: 60 mg/kg/7D- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
CRNGDP 21,2255,2000
subcutaneous/rat lowest published toxic dose: 100 mg/kg/5W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
CRNGDP 22,315,2000
subcutaneous/rat lowest published toxic dose: 105 mg/kg/5W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
CALEDQ 138,27,1999
subcutaneous/rat lowest published toxic dose: 160 mg/kg/4W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
MUREAV 506-507,129,2002
subcutaneous/rat lowest published toxic dose: 420 mg/kg/20W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Tumors
CALEDQ 147,125,1999
subcutaneous/rat toxic dose: 80 mg/kg/4W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
CRNGDP 6,637,1985
subcutaneous/rat toxic dose: 300 mg/kg/11W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
GASTAB 81,475,1981
subcutaneous/rat toxic dose: 42 mg/kg/28W- intermittentTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors

Gastrointestinal: Tumors
BIMDB3 32,41,1980
subcutaneous/rat toxic dose: 420 mg/kg/28W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Tumors

Kidney, Ureter, and Bladder: Kidney tumors
CRNGDP 4,1175,1983
subcutaneous/rat toxic dose: 200 mg/kg/20W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Gastrointestinal: Colon tumors
DICRAG 23,137,1980
subcutaneous/rat toxic dose: 400 mg/kg/10W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
LANCAO 2,1030,1980
subcutaneous/rat lowest published toxic dose: 105 mg/kg/24W- continuousTumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Other changes
CALEDQ 148,215,2000
subcutaneous/rat toxic dose: 160 mg/kg/2WTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Other changes
CALEDQ 154,121,2000
subcutaneous/rat lowest published toxic dose: 90 mg/kg/28W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Eye: Other eye effects

Gastrointestinal: Colon tumors
BIMDB3 32,41,1980
subcutaneous/rat lowest published toxic dose: 80 mg/kg/4W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors
CRNGDP 21,173,2000
subcutaneous/rat lowest published toxic dose: 160 mg/kg/2W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors
FCTOD7 50,2902,2012

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/rat Lowest published lethal concentration: 280 ppm/4HEye: Other eye effects

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Dyspnea
AMIHAB 12,609,1955
intramuscular/hamster lethal dose (50 percent kill): 95 mg/kgARZNAD 19,1891,1969
intraperitoneal/mouse lethal dose (50 percent kill): 35 mg/kgMEPAAX 24,71,1973
intraperitoneal/rat lethal dose (50 percent kill): 163 mg/kgMEPAAX 24,71,1973
intravenous/Dog lethal dose (50 percent kill): 100 mg/kgMEPAAX 24,71,1973
intravenous/mouse lethal dose (50 percent kill): 29 mg/kgMEPAAX 24,71,1973
intravenous/rat lethal dose (50 percent kill): 176 mg/kgMEPAAX 24,71,1973
oral/mouse lethal dose (50 percent kill): 36 mg/kgMEPAAX 24,71,1973
oral/rat lethal dose (50 percent kill): 100 mg/kgNATWAY 54,285,1967
oral/rat lowest published toxic dose: 10 mg/kgGastrointestinal: Necrotic changesFCTOD7 40,801,2002
oral/rat lowest published toxic dose: 25 mg/kgNutritional and Gross Metabolic: Weight loss or decreased weight gainFCTOD7 40,801,2002
subcutaneous/mouse lowest published toxic dose: 80 mg/kgGastrointestinal: Other changesJJIND8 93,1534,2001
subcutaneous/mouse lethal dose (50 percent kill): 24 mg/kgTOLED5 8,87,1981
subcutaneous/rat lethal dose (50 percent kill): 220 mg/kgXENOBH 3,271,1973
subcutaneous/rat lowest published toxic dose: 40 mg/kgLiver: Tumors

Gastrointestinal: Colon tumors
TXAPA9 227,136,2008

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse lowest published toxic dose: 136 mg/kg/20W- intermittentGastrointestinal: Colon tumorsCRNGDP 24,527,2003
intraperitoneal/mouse lowest published toxic dose: 360 mg/kg/12W- intermittentGastrointestinal: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Peptidases

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
FCTOD7 50,1405,2012
intraperitoneal/rat lowest published toxic dose: 120 mg/kg/6W- intermittentGastrointestinal: Colon tumors

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Catalases

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductases
EMMUEG 44,113,2004
intraperitoneal/rat lowest published toxic dose: 120 mg/kg/6W- intermittentGastrointestinal: Other changesEMMUEG 44,113,2004
subcutaneous/rat : 160 mg/kg/2W- intermittentGastrointestinal: Colon tumorsFCTOD7 44,877,2006
subcutaneous/rat lowest published toxic dose: 80 mg/kg/4W- intermittentGastrointestinal: Other changes

Gastrointestinal: Colon tumors

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 214,290,2006
subcutaneous/rat lowest published toxic dose: 180 mg/kg/6W- intermittentGastrointestinal: Colon tumorsBPBLEO 30,569,2007
subcutaneous/rat lowest published toxic dose: 60 mg/kg/5D- intermittentGastrointestinal: Other changesCRNGDP 24,491,2003
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15W- intermittentGastrointestinal: Tumors

Gastrointestinal: Colon tumors

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
FCTOD7 47,309,2009
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15W- intermittentGastrointestinal: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other transferases
FCTOD7 49,887,2011
subcutaneous/rat lowest published toxic dose: 480 mg/kg/16W- intermittentGastrointestinal: Colon tumors

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
TOLED5 171,10,2007
subcutaneous/rat lowest published toxic dose: 300 mg/kg/15W- intermittentGastrointestinal: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects

Biochemical: Metabolism (intermediary): Lipids including transport
PHMREP 47,133,2003

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 4,145,1974
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 71,947,1999
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 4,145,1974
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 71,947,1999
International Agency for Research on Cancer (IARC)Cancer Review:Group 2AIMEMDT 71,947,1999
TOXICOLOGY REVIEWMUREAV 39,111,1977
TOXICOLOGY REVIEWMUTAEX 15,431,2000
TOXICOLOGY REVIEWMUREAV 588,88,2005
TOXICOLOGY REVIEWEMMUEG 44,26,2004
TOXICOLOGY REVIEWFCTOD7 44,1699,2006
TOXICOLOGY REVIEWMUREAV 627,10,2007
TOXICOLOGY REVIEWBCLPT* 96,131,2005
TOXICOLOGY REVIEWJTPAE7 19,1,2006
TOXICOLOGY REVIEWMUREAV 658,68,2008
TOXICOLOGY REVIEWMUREAV 566,191,2004
TOXICOLOGY REVIEWHTOPA* 1,501,2002
TOXICOLOGY REVIEWMUREAV 705,40,2010
TOXICOLOGY REVIEWMUREAV 678,76,2009
TOXICOLOGY REVIEWMUTAEX 26,223,2011
TOXICOLOGY REVIEWEMMUEG 49,9,2008
TOXICOLOGY REVIEWMUREAV 591,276,2005
TOXICOLOGY REVIEWMUREAV 723,108,2011
TOXICOLOGY REVIEWEMMUEG 49,9,2008

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-HUNGARYceiling concentration 1.2 mg/m3, Carcinogen, SEP2000
Occupational Exposure Limit-SWEDENGroup B Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 0.5 ppm (1.2 mg/m3), carc 2, skin, sen, JAN2011

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO HYDRAZINES-airCarcinogen ceiling concentration 0.03 ppm/120MNIOSH* DHHS #92-100,1992

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/98/101025/AS)
EPA GENETOX PROGRAM 1988, Inconclusive: S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Inconclusive: SHE-clonal assay; Mammalian micronucleus
EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Host-mediated assay
EPA GENETOX PROGRAM 1988, Positive: E coli polA without S9
On EPA IRIS database
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