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Registry of Toxic Effects of Chemical Substances (RTECS)

tert-Butylhydroperoxide

RTECS #

EQ4900000

CAS #

75-91-2

Updated

December 2013

Molecular Weight

90.14

Molecular Formula

C4H10O2

Synonyms

1,1-Dimethylethyl hydroperoxide
BHP
Cadox TBH
Hydroperoxide, 1,1-dimethylethyl- (9CI)
Hydroperoxide, tert-butyl
Hydroperoxyde de butyle tertiaire (French)
Perbutyl H
TBHP-70
t-Butyl hydroperoxide
terc. Butylhydroperoxid (Czech)
Trigonox A-75 (Czech)
Trigonox A-W70

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 7 mgAIHAAP 19,205,1958
eye /rabbit 100 mg/24H moderate28ZPAK -,39,1972
eye /rabbit 150 mg/1M rinse severeZAARAM 8,25,1958
eye /rabbit 70 µL severeNTIS** OTS0543782
eye /rabbit 75%VCVGK* -,522,1994
eye /rabbit 0.02 mLVCVGK* -,522,1994
skin /rabbit 500 mgAIHAAP 19,205,1958
skin /rabbit 500 mg/24H severe28ZPAK -,39,1972

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisinhalation/rat107 mg/m3/10WGTPZAB 22(2),53,1978
Cytogenetic Analysisinhalation/mouse107 mg/m3/10WGTPZAB 22(2),53,1978
Cytogenetic Analysislung/hamster150 µmol/LMUREAV 213,243,1989
Cytogenetic Analysisinhalation/rat107.15 mg/m3/122DVCVGK* -,521,1994
Cytogenetic Analysisinhalation/rat107 µg/L/122D- intermittentTPKVAL 15,90,1979
Cytogenetic Analysislymphocyte/human500 µmol/L/30MMUREAV 559,97,2004
Cytogenetic Analysislymphocyte/human400 µmol/L/15MMUTAEX 21,21,2005
DNA Damagelymphocyte/human400 µmol/L/15MMUTAEX 21,21,2005
DNA Damageother cell types/human200 µmol/L/20MFCTOD7 46,2445,2008
DNA Damageother cell types/human100 µmol/L/1HTOLED5 177,66,2008
DNA Damageliver/human0.5 µmol/L/20MMUREAV 632,1,2007
DNA Damageovary/hamster100 µmol/L/1HMUREAV 583,189,2005
DNA Damageother cell types/rat0.1 mmol/L/1HMUREAV 535,103,2003
DNA Damageliver/rat0.05 mmol/L/1HMUREAV 535,103,2003
DNA Damage/Saccharomyes cerevisiae2 mmol/L/1HMUREAV 539,29,2003
DNA Damagelung/hamster500 µmol/LMUREAV 213,243,1989
DNA Damage/Salmonella typhimurium11 mmol/LEVHPAZ 88,111,1990
Dominant Lethal Testinhalation/mouse17 mg/m3/10WGTPZAB 22(2),53,1978
Dominant Lethal Testintraperitoneal/mouse1500 µmol/kg/5D-continuousMUREAV 444,145,1999
micronucleus testlymphocyte/human500 µmol/L/30MMUREAV 559,97,2004
mutation in microorganisms/Escherichia coli150 µg/plate/72HMUREAV 564,31,2004
mutation in microorganisms/Escherichia coli225 mg/L/24HMUREAV 564,31,2004
mutation in microorganisms/Salmonella typhimurium100 µg/plate/48H (+/-enzymatic activation step)MUREAV 539,29,2003
mutation in microorganisms/Escherichia coli25 µg/plate/48H (+/-enzymatic activation step)MUREAV 539,29,2003
mutation in microorganismsembryo/Salmonella typhimurium100 µg/plate/48HMUREAV 542,89,2003
mutation in microorganisms/Salmonella typhimurium45 µg/plate/72H (-enzymatic activation step)MUTAEX 15,495,2000
mutation in microorganisms/Escherichia coli20 µg/plate/48HMUTAEX 15,317,2000
mutation in microorganisms/Salmonella typhimurium20 µg/plate/48HMUTAEX 15,317,2000
mutation in microorganisms/Escherichia coli50 µg/plate/20MMUREAV 492,91,2001
mutation in microorganisms/Escherichia coli78 µg/plate (-enzymatic activation step)MUREAV 412,17,1998
mutation in microorganisms/Escherichia coli25 µg/plate (+enzymatic activation step)MUREAV 467,41,2000
mutation in microorganisms/Salmonella typhimurium17 µg/plate (+/-enzymatic activation step)ENMUDM 5(Suppl 1),3,1983
mutation in microorganisms/Neurospora crassa10 mmol/L/30M (-enzymatic activation step)PNASA6 35,581,1949
mutation in microorganisms/Salmonella typhimurium1 µmol/plate/48HMUREAV 527,27,2003
mutation in microorganisms/Escherichia coli50 µg/plate/2DMUREAV 539,187,2003
mutation in microorganisms/Salmonella typhimurium1 µmol/plate/73H (+/-enzymatic activation step)MUREAV 629,122,2007
other mutation test systems/Escherichia coli30 µmol/LMUREAV 203,81,1988
phage inhibition capacity/Escherichia coli25 mg/LVIRLAX 99,257,1979
sex chromosome loss and nondisjunctioninhalation/Drosophila melanogaster500 mmol/LPNASA6 40,1037,1954
sex chromosome loss and nondisjunctionparenteral/Drosophila melanogaster1000 ppmENMUDM 7,677,1985
sex chromosome loss and nondisjunctionlung/hamster34 µmol/LMUREAV 182,135,1987
specific locus testmultiple/Drosophila melanogaster1 mmol/LMUREAV 440,139,1999
sperm morphologyinhalation/mouse107 µg/L/122D- intermittentTPKVAL 15,90,1979
Unscheduled DNA Synthesisliver/rat1.5 mmol/L/30MARTODN 79,102,2005

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse 1059.8 mg/kg (2W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth)
REPTED 22,479,2006

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, leukemia cells Inhibitor Concentration Low: 4 mmol/L/3HBiochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductasesTXCYAC 278,242,2010
In Vitro/Human, liver tumor Inhibitor Concentration Low: 300 µmol/L/24HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)

In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Apoptosis in vitro
FCTOD7 49,2081,2011
In Vitro/Human, liver tumor Inhibitor Concentration Low: 300 µmol/L/6HIn Vitro Toxicity Studies: Other assaysFCTOD7 49,2081,2011
In Vitro/Human, liver tumor Inhibitor Concentration Low: 400 µmol/LIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductases

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other transferases
FCTOD7 50,2886,2012
In Vitro/Human, liver tumor Inhibitor Concentration Low: 400 µmol/LIn Vitro Toxicity Studies: Other assaysFCTOD7 50,2886,2012
In Vitro/Human, melanoma Inhibitor Concentration Low: 300 µmol/LIn Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.

In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Apoptosis in vitro
FCTOD7 50,1781,2012
In Vitro/Human, melanoma Inhibitor Concentration Low: 200 µmol/L/1HIn Vitro Toxicity Studies: Other assaysFCTOD7 50,2171,2012
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 25 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.NRTXDN 33,138,2012
In Vitro/jrdInhibitor Concentration Low: 50 µmol/L/24HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)PYTOEY 18,1238,2011
In Vitro/jrdInhibitor Concentration Low: 50 µmol/L/30MIn Vitro Toxicity Studies: Other assaysPYTOEY 18,1238,2011
In Vitro/jrdInhibitor Concentration Low: 50 µmol/L/2HBiochemical: Metabolism (intermediary): Effect on mitochondrial functionPYTOEY 18,1238,2011
In Vitro/jtiInhibitor Concentration Low: 300 µmol/L/30MIn Vitro Toxicity Studies: Other assaysFTRPAE 83,130,2012
In Vitro/mzxInhibitor Concentration Low: 150 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.NRTXDN 33,272,2012
In Vitro/qpbInhibitor Concentration Low: 2 mmol/L/30MIn Vitro Toxicity Studies: Other assaysFCTOD7 49,2212,2011
In Vitro/Rat, endocrine tumor Inhibitor Concentration Low: 75 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.NRTXDN 33,138,2012
inhalation/mouse lethal concentration (50 percent kill): 350 ppm/4HLung, Thorax, or Respiration: DyspneaAIHAAP 19,205,1958
inhalation/mouse lethal concentration (50 percent kill): 1800 mg/m3/4HVCVGK* -,520,1994
inhalation/rat lethal concentration (50 percent kill): 1800 mg/m3/4HVCVGK* -,520,1994
inhalation/rat lowest published toxic concentration: 600 mg/m3/4HBehavioral: Alteration of classical conditioning

Lung, Thorax, or Respiration: Dyspnea

Blood: Methemoglobinemia- Carboxhemoglobinemia
VCVGK* -,520,1994
inhalation/rat lowest published toxic concentration: 180 mg/m3Olfaction: Change in sensation of smell

Lung, Thorax, or Respiration: Dyspnea
VCVGK* -,520,1994
inhalation/rat lethal concentration (50 percent kill): 500 ppm/4HLung, Thorax, or Respiration: DyspneaAIHAAP 19,205,1958
inhalation/rat lowest published toxic concentration: 260 mg/m3/4HLung, Thorax, or Respiration: Other changesTPKVAL 14,73,1975
inhalation/rat lowest published toxic concentration: 180 mg/m3Behavioral: TremorTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 260 mg/m3Behavioral: Somnolence (general depressed activity)TPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 360 mg/m3TPKVAL 15,90,1979
intraperitoneal/mouse lowest published toxic dose: 5408.4 mg/kgBiochemical: Enzyme inhibition, induction, or change in blood or tissue levels: TransaminasesBPBLEO 28,531,2005
intraperitoneal/mouse lowest published toxic dose: 180.28 mg/kgLiver: Hepatitis (hepatocellular necrosis), zonal

Liver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
FCTOD7 46,3512,2008
intraperitoneal/mouse lethal dose (50 percent kill): 2400 µg/kgVCVGK* -,521,1994
intraperitoneal/rat lethal dose (50 percent kill): 87 mg/kgBehavioral: Muscle weakness

Behavioral: Ataxia
AIHAAP 19,205,1958
intraperitoneal/rat lowest published toxic dose: 20 mg/kgLiver: Hepatitis (hepatocellular necrosis), zonal

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
TOLED5 147,271,2004
intraperitoneal/rat lowest published toxic dose: 9.014 mg/kgLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases

Biochemical: Metabolism (intermediary): Lipids including transport
ARTODN 76,664,2002
intraperitoneal/rat lowest published toxic dose: 9.014 mg/kgLiver: Hepatitis (hepatocellular necrosis), zonal

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects

Biochemical: Metabolism (intermediary): Lipids including transport
ARTODN 79,102,2005
intraperitoneal/rat lethal dose (50 percent kill): 87 mg/kgENTOX* -,554,2005
intraperitoneal/rat lowest published toxic dose: 18.028 mg/kgLiver: Hepatitis (hepatocellular necrosis), zonal

Liver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
FCTOD7 49,2081,2011
intraperitoneal/rat lowest published toxic dose: 9014 mg/kgLiver: Hepatitis (hepatocellular necrosis), diffuse

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Dehydrogenases

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
FCTOD7 50,1215,2012
intraperitoneal/rat lowest published toxic dose: 19.028 mg/kgLiver: Other changesFCTOD7 50,1215,2012
intraperitoneal/rat lowest published toxic dose: 45.07 mg/kgLiver: Hepatitis (hepatocellular necrosis), diffuseFCTOD7 50,1215,2012
intraperitoneal/rat lowest published toxic dose: 9.014 mg/kgLiver: Hepatitis (hepatocellular necrosis), diffuse

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
FCTOD7 40,635,2002
intraperitoneal/rat lowest published toxic dose: 9.014 mg/kgLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
BPBLEO 28,1639,2005
intraperitoneal/rat lowest published toxic dose: 180.28 mg/kgLiver: Hepatitis (hepatocellular necrosis), diffuse

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases

Biochemical: Metabolism (intermediary): Lipids including transport
FCTOD7 46,2445,2008
intravenous/rat lowest published lethal dose: 20 mg/kgVCVGK* -,521,1994
oral/cat lowest published toxic dose: 500 mg/kgBlood: Methemoglobinemia- Carboxhemoglobinemia

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)
VCVGK* -,521,1994
oral/mouse lethal dose (50 percent kill): 320 mg/kgVCVGK* -,521,1994
oral/mouse lethal dose (50 percent kill): 320 mg/kgBehavioral: Irritability

Gastrointestinal: Alteration in gastric secretion

Blood: Hemorrhage
TPKVAL 15,90,1979
oral/rat lethal dose (50 percent kill): 370 mg/kgBehavioral: Irritability

Gastrointestinal: Alteration in gastric secretion

Blood: Hemorrhage
TPKVAL 15,90,1979
oral/rat lethal dose (50 percent kill): 370 mg/kgVCVGK* -,521,1994
skin/rabbit lethal dose (50 percent kill): 460 µL/kgLung, Thorax, or Respiration: Cyanosis

Liver: Other changes

Kidney, Ureter, and Bladder: Other changes in urine composition
ARCOC* JUN1982
skin/rat lethal dose (50 percent kill): 790 mg/kgBSPII* 1/75-19B
subcutaneous/rat lowest published toxic dose: 800 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaVCVGK* -,521,1994
subcutaneous/rat lowest published toxic dose: 117 mg/kgLiver: Other changes

Blood: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Catalases
FCTOD7 42,1315,2004

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/guinea pig lowest published toxic concentration: 107 mg/m3/61D- intermittentBlood: Changes in erythrocyte (RBC) count

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
TPKVAL 15,90,1979
inhalation/guinea pig lowest published toxic concentration: 17.3 mg/m3/61D- intermittentBlood: Other changesTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 107 mg/m3/61D- intermittentPeripheral Nerve and Sensation: Recording from peripheral motor nerve

Liver: Liver function tests impaired

Kidney, Ureter, and Bladder: Proteinuria
TPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 107 mg/m3/91D- intermittentOlfaction: Change in sensation of smellTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 107 mg/m3/30D- intermittentBlood: Methemoglobinemia- CarboxhemoglobinemiaTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 17.3 mg/m3/30D- intermittentKidney, Ureter, and Bladder: Other changes in urine compositionTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 17.3 mg/m3/61D- intermittentLung, Thorax, or Respiration: Respiratory depressionTPKVAL 15,90,1979
inhalation/rat lowest published toxic concentration: 17.3 mg/m3/122D- intermittentOlfaction: Change in sensation of smell

Lung, Thorax, or Respiration: Other changes
TPKVAL 15,90,1979
intraperitoneal/mouse lowest published toxic dose: 1059.8 mg/kg/2W- intermittentReproductive: Other effects on male

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects

Biochemical: Metabolism (intermediary): Lipids including transport
REPTED 22,479,2006
intraperitoneal/rat lowest published toxic dose: 365 mg/kg/7W- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldAIHAAP 19,205,1958

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWENTOX* -,554,2005
TOXICOLOGY REVIEWMUREAV 658,28,2008
TOXICOLOGY REVIEWMUTAEX 23,153,2008
TOXICOLOGY REVIEWMUREAV 704,115,2010
TOXICOLOGY REVIEWMUREAV 728,1,2011

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-RUSSIAshort term exposure limit 5 mg/m3, Skin, JUN2003

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code 14730; Number of Industries 8; Total Number of Facilities 266; Number of Occupations 14; Total Number of Employees Exposed 12168; Total Number of Female Employees Exposed 4787
National Occupational Hazard Survey 1974Hazard Code 14730; Number of Industries 2; Total Number of Facilities 75; Number of Occupations 3; Total Number of Employees Exposed 433

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Positive: N crassa-reversion; S cerevisiae gene conversion
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
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