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Registry of Toxic Effects of Chemical Substances (RTECS)

Benzene, 1-chloro-2-nitro-

RTECS #

CZ0875000

CAS #

88-73-3

Updated

November 2009

Molecular Weight

157.56

Molecular Formula

C6H4ClNO2

Synonyms

1-Chloro-2-nitrobenzene
1-Nitro-2-chlorobenzene
2-Chloro-1-nitrobenzene
2-Chloronitrobenzene
Chloro-o-nitrobenzene
o-Chloronitrobenzene
ONCB
o-Nitrochlorobenzene

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisovary/hamster465000 µg/L/14H (+/-enzymatic activation step)HPV175 -,-,2003
mutation in microorganisms/Salmonella typhimurium205 µg/plate (+enzymatic activation step)MUREAV 116,217,1983
mutation in microorganisms/Salmonella typhimurium100 µg/plate (-enzymatic activation step)ENMUDM 5(Suppl 1),3,1983

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
inhalation/rat 18 ppm/6H (13W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)NTPTR* NIH-93-3382
inhalation/mouse 4500 ppb/6H (13W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)NTPTR* NIH-93-3382
oral/mouse 16800 mg/kg (105D male/105D prior to copulation)Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)NTIS** PB92-187608/AS
oral/rat 750 mg/kg (6-15D pregnant)Reproductive: Other effects on femaleHPV175 -,-,2003

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 140 gm/kg/78W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
JEPTDQ 2(2),325,1978
oral/mouse toxic dose: 280 gm/kg/78W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
JEPTDQ 2(2),325,1978
oral/rat lowest published toxic dose: 22 gm/kg/78W- continuousTumorigenic: Neoplastic by RTECS criteria

Gastrointestinal: Tumors

Endocrine: Tumors
JEPTDQ 2(2),325,1978

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
oral/mouse lethal dose (50 percent kill): 135 mg/kgNTIS** PB214-270
oral/rabbit lethal dose (50 percent kill): 280 mg/kg85GMAT -,92,1982
oral/rat lethal dose (50 percent kill): 268 mg/kgNTIS** PB214-270
oral/rat lethal dose (50 percent kill): 560 mg/kgLung, Thorax, or Respiration: Other changes

Liver: Other changes

Blood: Change in clotting factors
HPV175 -,-,2003
skin/rabbit lethal dose (50 percent kill): 400 mg/kgFAATDF 7,609,1986

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/mouse lowest published toxic concentration: 18 ppm/6H/2W- continuousLiver: Hepatitis (hepatocellular necrosis), zonal

Kidney, Ureter, and Bladder: Changes in bladder weight

Endocrine: Changes in spleen weight
NTPTR* NIH-93-3382
inhalation/mouse lowest published toxic concentration: 2300 ppb/6H/13W- intermittentLiver: Changes in liver weight

Kidney, Ureter, and Bladder: Changes in bladder weight
NTPTR* NIH-93-3382
inhalation/rat lowest published toxic concentration: 1.1 ppm/13W- intermittentLung, Thorax, or Respiration: Other changes

Blood: Methemoglobinemia- Carboxhemoglobinemia

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Dehydrogenases
HPV175 -,-,2003
inhalation/rat lowest published toxic concentration: 30 mg/m3/4W- intermittentLiver: Changes in liver weight

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in spleen
HPV175 -,-,2003
inhalation/rat lowest published toxic concentration: 30 mg/m3/6H/4W- intermittentLiver: Changes in liver weight

Endocrine: Changes in spleen weight

Blood: Methemoglobinemia- Carboxhemoglobinemia
FAATDF 7,609,1986
inhalation/rat lowest published toxic concentration: 4500 ppb/6H/13W- continuousLiver: Changes in liver weight

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in erythrocyte (RBC) count
NTPTR* NIH-93-3382
inhalation/rat lowest published toxic concentration: 530 mg/m3/6H/2W- intermittentBlood: Other hemolysis with or without anemia

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in erythrocyte (RBC) count
NTIS** OTS0546562
oral/mouse lowest published toxic dose: 16800 mg/kg/105D- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gain

Endocrine: Changes in spleen weight

Blood: Methemoglobinemia- Carboxhemoglobinemia
NTIS** PB92-187608/AS
oral/mouse lowest published toxic dose: 16800 mg/kg/27W- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
NTIS** PB92-187608/AS
oral/mouse lowest published toxic dose: 39420 mg/kg/2Y- continuousLiver: Changes in liver weight

Kidney, Ureter, and Bladder: Changes in kidney weight

Endocrine: Changes in spleen weight
JTSCDR 31,247,2006
oral/mouse lowest published toxic dose: 8030 mg/kg/2Y- continuousLiver: Other changes

Liver: Tumors

Blood: Changes in spleen
JTSCDR 31,247,2006
oral/mouse lowest published toxic dose: 10220 mg/kg/2Y- continuousLiver: TumorsJTSCDR 31,247,2006
oral/mouse lowest published toxic dose: 31404.1 mg/kg/13W- continuousLiver: Changes in liver weightJTSCDR 31,9,2006
oral/mouse lowest published toxic dose: 15506.4 mg/kg/13W- continuousBlood: Other changes

Blood: Changes in erythrocyte (RBC) count

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
JTSCDR 31,9,2006
oral/mouse lowest published toxic dose: 17881.5 mg/kg/13W- continuousLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JTSCDR 31,9,2006
oral/mouse lowest published toxic dose: 4504.5 mg/kg/13W- continuousBlood: Changes in spleen

Blood: Other changes
JTSCDR 31,9,2006
oral/mouse lowest published toxic dose: 3967.6 mg/kg/13W- continuousLiver: Other changes

Blood: Changes in spleen
JTSCDR 31,9,2006
oral/mouse lowest published toxic dose: 69387.5 mg/kg/13W- continuousBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 1255.8 mg/kg/13W- continuousBlood: Changes in spleen

Blood: Other changes
JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 1410.5 mg/kg/13W- continuousBlood: Changes in spleenJTSCDR 31,9,2006
oral/rat lowest published toxic dose: 5232.5 mg/kg/13W- continuousLiver: Other changes

Blood: Changes in erythrocyte (RBC) count

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 5814.9 mg/kg/13W- continuousLiver: Other changesJTSCDR 31,9,2006
oral/rat lowest published toxic dose: 364 mg/kg/13W- continuousBlood: Other changes

Blood: Changes in erythrocyte (RBC) count
JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 12130.3 mg/kg/13W- continuousBlood: Changes in bone marrow not included in above

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 10874.5 mg/kg/13W- continuousBlood: Changes in bone marrow not included in aboveJTSCDR 31,9,2006
oral/rat lowest published toxic dose: 21303.1 mg/kg/13W- continuousLiver: Changes in liver weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
JTSCDR 31,9,2006
oral/rat lowest published toxic dose: 52668 mg/kg/76W- continuousRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldJTSCDR 31,247,2006
oral/rat lowest published toxic dose: 13860 mg/kg/20W- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainJTSCDR 31,247,2006
oral/rat lowest published toxic dose: 13870 mg/kg/2Y- continuousLiver: Other changes

Liver: Tumors

Kidney, Ureter, and Bladder: Changes in kidney weight
JTSCDR 31,247,2006
oral/rat lowest published toxic dose: 2920 mg/kg/2Y- continuousLiver: Changes in liver weight

Blood: Other changes
JTSCDR 31,247,2006
oral/rat lowest published toxic dose: 16060 mg/kg/2Y- continuousLiver: Changes in liver weight

Kidney, Ureter, and Bladder: Other changes

Kidney, Ureter, and Bladder: Changes in kidney weight
JTSCDR 31,247,2006
oral/rat lowest published toxic dose: 85410 mg/kg/2Y- continuousLiver: Other changes

Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
JTSCDR 31,247,2006
oral/rat lowest published toxic dose: 289080 mg/kg/2Y- continuousLiver: Tumors

Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Blood: Changes in erythrocyte (RBC) count
JTSCDR 31,247,2006
oral/rat lowest published toxic dose: 1050 mg/kg/28W- intermittentKidney, Ureter, and Bladder: Other changes in urine composition

Blood: Methemoglobinemia- Carboxhemoglobinemia

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases
HPV175 -,-,2003
oral/rat lowest published toxic dose: 1500 mg/kg/10D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldHPV175 -,-,2003

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Inadequate EvidenceIMEMDT 65,263,1996
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 65,263,1996
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMEMDT 65,263,1996

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-POLANDMAC(time-weighted average) 1 mg/m3, MAC(short term exposure limit) 3 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAshort term exposure limit 1 mg/m3, Skin, JUN2003

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code X4011; Number of Industries 3; Total Number of Facilities 42; Number of Occupations 5; Total Number of Employees Exposed 2897; Total Number of Female Employees Exposed 662

Status in Federal Agencies

OrganizationReference
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA Section 8(e) Risk Notification, 8EHQ-0892-9198
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NTP Toxicity studies, RPT# TOX-33, October 2000
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