Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to page options Skip directly to site content

Registry of Toxic Effects of Chemical Substances (RTECS)

Benzene, 1-chloro-2,4-dinitro-

RTECS #

CZ0525000

CAS #

97-00-7

Updated

December 2013

Molecular Weight

202.56

Molecular Formula

C6H3ClN2O4

Synonyms

1,3-Dinitro-4-chlorobenzene
1-Chloor-2,4-dinitrobenzeen (Dutch)
1-Chlor-2,4-dinitrobenzene
1-Chloro-2,4-dinitrobenzene
1-Chloro-2,4-dinitrobenzol (German)
1-Cloro-2,4-dinitrobenzene (Italian)
2,4-Dinitro-1-chlorobenzene
2,4-Dinitrochlorobenzene
4-Chloro-1,3-dinitrobenzene
6-Chloro-1,3-dinitrobenzene
CDNB
Dinitrochlorobenzol
DNCB

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 50 µg/24H severe85JCAE -,600,1986
skin /human 0.3%/24HADVEA4 94,407,1976
skin /human 0.3%/48HADVEA4 94,407,1976
skin /human 30 µgCODEDG 2,247,1976
skin /mouse 0.3%/24H open irritation test mildFCTOD7 40,1713,2002
skin /rabbit 100 µg/24H open irritation testAIHAAP 23,95,1962
skin /rabbit 2 mg/24H severe85JCAE -,600,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
DNA Damageliver/rat5 µmol/LMUREAV 131,215,1984
DNA Damageintraperitoneal/mouse30 mg/kgMUREAV 116,239,1983
morphological transformkidney/hamster10 mg/LARTODN 45,307,1980
mutation in microorganisms/Salmonella typhimurium25 µg/plate (+/-enzymatic activation step)MUREAV 520,119,2002
mutation in microorganisms/Salmonella typhimurium3 µg/plate (+enzymatic activation step)ARDEAC 121,348,1985
mutation in microorganisms/Salmonella typhimurium50 µg/plate (-enzymatic activation step)ADREDL 266,315,1979

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, leukemia cells Inhibitor Concentration Low: 3 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc.TXCYAC 278,359,2010
In Vitro/Human, leukemia cells Inhibitor Concentration Low: 30 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TXCYAC 278,359,2010
In Vitro/Human, monocyte Inhibitor Concentration Low: 30 µmol/L/48HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Other assays

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 246,66,2010
In Vitro/Human, monocyte Inhibitor Concentration Low: 15 µmol/L/48HIn Vitro Toxicity Studies: Other assays

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 246,66,2010
In Vitro/Human, monocyte Inhibitor Concentration Low: 15 µmol/L/30MBiochemical: Metabolism (intermediary): Effect on mitochondrial functionTXAPA9 246,66,2010
In Vitro/Human, monocyte Inhibitor Concentration Low: 7.5 µmol/L/15MIn Vitro Toxicity Studies: Other assaysTXCYAC 274,1,2010
In Vitro/Human, monocyte Inhibitor Concentration Low: 15 µmol/L/3HNutritional and Gross Metabolic: Changes in: CaTXCYAC 274,1,2010
intraperitoneal/rat lethal dose (50 percent kill): 280 mg/kgBehavioral: Convulsions or effect on seizure threshold

Gastrointestinal: Peritonitis

Blood: Methemoglobinemia- Carboxhemoglobinemia
AGGHAR 17,217,1959
oral/rat lethal dose (50 percent kill): 640 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaAGGHAR 17,217,1959
skin/guinea pig lowest published toxic dose: 60 mg/kgSkin: After topical application: Cutaneous sensitization (experimental)ADSYAF 76,549,1957
skin/rabbit lethal dose (50 percent kill): 130 mg/kgSkin: After topical application: Primary irritationAIHAAP 23,95,1962

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/mouse lowest published toxic concentration: 30 mg/m3/3D- intermittentImmunological Including Allergic: Increased immune response

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXCYAC 261,103,2009
inhalation/rat lowest published toxic concentration: 200 µg/m3/4H/17W- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Other proteins
GTPZAB 29(8),59,1985
oral/rat lowest published toxic dose: 2340 mg/kg/30D- intermittentBrain and Coverings: Other degenerative changes

Liver: Other changes
GTPZAB 32(2),48,1988
skin/mouse lowest published toxic dose: 1 pph/3D- intermittentImmunological Including Allergic: Increased immune response

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXCYAC 261,103,2009
skin/mouse lowest published toxic dose: 10 pph/3D- intermittentImmunological Including Allergic: Increased immune responseTXCYAC 261,152,2009
skin/mouse lowest published toxic dose: 60 mg/kg/3D- intermittentSkin: After topical application: Dermatitis, allergic

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TOLED5 190,231,2009
skin/mouse lowest published toxic dose: 624 mg/kg/9W- intermittentSkin: After topical application: Dermatitis, allergicFCTOD7 50,2923,2012
skin/mouse lowest published toxic dose: 67.2 mg/kg/13D- intermittentImmunological Including Allergic: Increase in cellular immune response

Immunological Including Allergic: Hypersensitivity delayed (multiple organ involvement)
JJATDK 20,221,2000
skin/mouse lowest published toxic dose: 2.5 mg/kg/5D- intermittentImmunological Including Allergic: Increase in cellular immune response

Tumorigenic: Cells (cultured) transformed
JAPTO* 21,257,2001
skin/mouse lowest published toxic dose: 60 mg/kg/7D- intermittentImmunological Including Allergic: Increase in cellular immune response

Immunological Including Allergic: Increase in humoral response
JJATDK 22,397,2002
skin/mouse lowest published toxic dose: 15 mg/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Immunological Including Allergic: Hypersensitivity delayed (multiple organ involvement)

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 183,66,2002
skin/mouse lowest published toxic dose: 15 mg/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 184,46,2002
skin/mouse lowest published toxic dose: 6 mg/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)FCTOD7 40,1713,2002
skin/mouse lowest published toxic dose: 70 mg/kg/8D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Immunological Including Allergic: Increase in cellular immune response
FCTOD7 40,1881,2002
skin/mouse lowest published toxic dose: 6 mg/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Immunological Including Allergic: Increase in cellular immune response
TXCYAC 190,259,2003
skin/mouse lowest published toxic dose: 40 mg/kg/3D- intermittentImmunological Including Allergic: Hypersensitivity delayed (multiple organ involvement)

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
ETOPFR 14,61,2003
skin/mouse lowest published toxic dose: 15 mg/kg/3D- intermittentImmunological Including Allergic: Increase in cellular immune responseETOPFR 17,95,2004
skin/mouse lowest published toxic dose: 60 mg/kg/3D- intermittentImmunological Including Allergic: Increase in cellular immune response

Immunological Including Allergic: Increase in humoral response
TXCYAC 206,221,2005
skin/mouse lowest published toxic dose: 9 mg/kg/3D- continuousImmunological Including Allergic: Increase in cellular immune responseTXCYAC 232,132,2007
skin/mouse lowest published toxic dose: 240 mg/kg/28D- intermittentImmunological Including Allergic: Increase in humoral responseTOSCF2 94,108,2006
skin/mouse lowest published toxic dose: 0.03 mL/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)TOLED5 177,1,2008
skin/mouse lowest published toxic dose: 6 ul/kg/3D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)TOLED5 180,1,2008
skin/mouse lowest published toxic dose: 54 ul/kg/17D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)TOLED5 180,1,2008
skin/mouse lowest published toxic dose: 54.6 ul/kg/31D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Immunological Including Allergic: Increased immune response
TOLED5 180,1,2008
skin/mouse lowest published toxic dose: 60 mg/kg/3D- intermittentImmunological Including Allergic: Increase in humoral response

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXCYAC 250,116,2008
skin/mouse lowest published toxic dose: 110 mg/kg/8D- intermittentImmunological Including Allergic: Increase in cellular immune response

Biochemical: Metabolism (intermediary): Other proteins

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXCYAC 252,17,2008
skin/rat lowest published toxic dose: 20.6 mg/kg/8D- intermittentSkin: After topical application: Cutaneous sensitization (experimental)

Immunological Including Allergic: Increase in cellular immune response
FCTOD7 40,1881,2002

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWTOLED5 140-141,213,2003
TOXICOLOGY REVIEWMUREAV 682,39,2009
TOXICOLOGY REVIEWMUREAV 705,40,2010

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-JAPANs1 sen, MAY2012
Occupational Exposure Limit-RUSSIAtime-weighted average 0.05 mg/m3, short term exposure limit 0.2 mg/m3, Skin, JUN2003

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code 83616; Number of Industries 2; Total Number of Facilities 82; Number of Occupations 2; Total Number of Employees Exposed 170; Total Number of Female Employees Exposed 14
National Occupational Hazard Survey 1974Hazard Code 83616; Number of Industries 1; Total Number of Facilities 174; Number of Occupations 1; Total Number of Employees Exposed 1221

Status in Federal Agencies

OrganizationReference
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
TOP