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Registry of Toxic Effects of Chemical Substances (RTECS)

Benzene, 1,2-dichloro-4-nitro-

RTECS #

CZ5250000

CAS #

99-54-7

Updated

November 2009

Molecular Weight

192.00

Molecular Formula

C6H3Cl2NO2

Synonyms

1,2-Dichloro-4-nitrobenzene
3,4-Dichlornitrobenzen (Czech)
3,4-Dichloronitrobenzene
DCNB
Nitrated orthene

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 100 mg/24H moderate85JCAE -,601,1986
skin /rabbit 500 mg/24H mild85JCAE -,601,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
mutation in microorganisms/Salmonella typhimurium250 µg/plate (+/-enzymatic activation step)SAIGBL 29,34,1987
sex chromosome loss and nondisjunctionparenteral/Drosophila melanogaster200 ppmENMUDM 7,677,1985

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/rat 1500 mg/kg (6-12D pregnant)Reproductive: Other effects on femaleNTIS** OTS0534376

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/rat lethal concentration (50 percent kill): 10 gm/m3/4HOlfaction: Other olfaction effects

Behavioral: Somnolence (general depressed activity)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
NTIS** OTS0533734
intraperitoneal/mouse lethal dose (50 percent kill): 400 mg/kgBehavioral: Somnolence (general depressed activity)

Behavioral: Muscle weakness

Lung, Thorax, or Respiration: Respiratory depression
NTIS** OTS0533585
intraperitoneal/rat lethal dose (50 percent kill): 400 mg/kgBehavioral: Somnolence (general depressed activity)

Behavioral: Muscle weakness

Lung, Thorax, or Respiration: Cyanosis
NTIS** OTS0533585
oral/guinea pig lethal dose (50 percent kill): 500 mg/kgNTIS** OTS0536149
oral/mouse lowest published toxic dose: 500 mg/kgBlood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)
TOXID9 -,45,2008
oral/mouse lethal dose (50 percent kill): 1384 mg/kg85GMAT -,47,1982
oral/rat lethal dose (50 percent kill): 953 mg/kgNutritional and Gross Metabolic: Weight loss or decreased weight gainNTIS** OTS0533734
skin/cat lethal dose (50 percent kill): 790 mg/kg85GMAT -,47,1982
skin/rabbit Lethal dose: >200 mg/kgNutritional and Gross Metabolic: Weight loss or decreased weight gainNTIS** OTS0533734

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/mouse lowest published toxic concentration: 28 mg/m3/4H/21D- intermittentBlood: Eosinophilia

Blood: Changes in erythrocyte (RBC) count

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Catalases
GISAAA 34(11),26,1969
inhalation/rat lowest published toxic concentration: 10 mg/m3/4H/17W- intermittentBlood: Thrombocytopenia

Blood: Changes in erythrocyte (RBC) count

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases
GISAAA 34(11),26,1969
oral/mouse lowest published toxic dose: 2100 mg/kg/14D- intermittentBlood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)
TOXID9 -,45,2008
oral/mouse lowest published toxic dose: 2100 mg/kg/14D- intermittentBlood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in spleen

Blood: Changes in other cell count (unspecified)
TOXID9 -,45,2008
oral/rat lowest published toxic dose: 4200 mg/kg/4W- continuousKidney, Ureter, and Bladder: Inflammation, necrosis or scarring of bladder

Endocrine: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
NTIS** OTS0534360

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWFCTOD7 47,1287,2009

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-RUSSIAtime-weighted average 1 mg/m3, short term exposure limit 3 mg/m3, Skin, JUN2003

Status in Federal Agencies

OrganizationReference
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA Section 8(e) Risk Notification, 8EHQ-0892-9189
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
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