Methane, trichloronitro-

RTECS #
PB6300000
CAS #
76-06-2
See: NMAM or OSHA Methods
Updated
December 2018
Molecular Weight
164.37
Molecular Formula
CCl3NO2
Synonyms
Acquinite
Chloorpikrine (Dutch)
Chloroform, nitro-
Chloropicrin
Chloropicrin (ACGIH:OSHA)
Chloropicrine (French)
Chlorpikrin (German)
Cloropicrina (Italian)
Dojyopicrin
Dolochlor
G 25
KLOP
Larvacide
Microlysin
NCI-C00533
Nitrochloroform
Nitrotrichloromethane (OSHA)
Pic-Clor
Picfume
Picride
Profume A
PS
Trichloornitromethaan (Dutch)
Trichlornitromethan (German)
Trichloronitromethane
Tri-clor
Tricloro-nitro-metano (Italian)

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
mutation in microorganisms/Salmonella typhimurium50 µg/plate (-enzymatic activation step)MUREAV 116,185,1983
mutation in microorganisms/Escherichia coli50 µg/plate (+enzymatic activation step)SAIGBL 29,34,1987
other mutation test systems/Escherichia coli300 µg/LMUREAV 348,147,1995
sister chromatid exchangelymphocyte/human8 mg/LTCMUD8 10,21,1990

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 26 gm/kg/78W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors
NCITR* NCI-TR-65,1978

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, lung Inhibitor Concentration (50 percent kill): 111 µmol/L/24HIn Vitro Toxicity Studies: Cell countingTIVIEQ 45,72,2017
In Vitro/Human, lung Inhibitor Concentration Low: 10 µmol/L/24HIn Vitro Toxicity Studies: Cell morphology: overgrowth of cell appendixes etc.TIVIEQ 42,247,2017
In Vitro/Human, lung fibroblast Inhibitor Concentration Low: 16 µmol/L/6HIn Vitro Toxicity Studies: Other assaysTIVIEQ 28,209,2014
In Vitro/Human, lung tumor Inhibitor Concentration Low: 50 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Apoptosis in vitro
TOLED5 226,236,2014
In Vitro/Human, lung tumor Inhibitor Concentration (50 percent kill): 112 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TOLED5 226,236,2014
In Vitro/jdaInhibitor Concentration Low: 50 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Other assays
TOLED5 211,239,2012
inhalation/cat Lowest published lethal concentration: 800 mg/m3/20MBehavioral: Food intake (animal)

Lung, Thorax, or Respiration: Acute pulmonary edema

Lung, Thorax, or Respiration: Dyspnea
ZGEMAZ 106,296,1939
inhalation/Dog Lowest published lethal concentration: 0.5 gm/m3/30MCardiac: Cardiomyopathy including infarction

Lung, Thorax, or Respiration: Changes in pulmonary vascular resistance

Lung, Thorax, or Respiration: Respiratory obstruction
TPKVAL 3,103,1961
inhalation/guinea pig Lowest published lethal concentration: 0.5 gm/m3/30MBrain and Coverings: Changes in circulation

Behavioral: Ataxia

Behavioral: Tetany
TPKVAL 3,103,1961
inhalation/guinea pig Lowest published lethal concentration: 800 mg/m3/20MLung, Thorax, or Respiration: Emphysema

Lung, Thorax, or Respiration: Acute pulmonary edema

Lung, Thorax, or Respiration: Dyspnea
ZGEMAZ 106,296,1939
inhalation/human lowest published toxic concentration: 2 mg/m3Eye: Lacrimation

Eye: Conjunctiva irritation

Lung, Thorax, or Respiration: Other changes
ZGEMAZ 106,296,1939
inhalation/human Lowest published lethal concentration: 2000 mg/m3/10M34ZIAG -,169,1969
inhalation/mouse lethal concentration (50 percent kill): 66 mg/m3/4HEye: Increased intraocular pressure

Behavioral: Somnolence (general depressed activity)

Lung, Thorax, or Respiration: Respiratory stimulation
SAIGBL 15,406,1973
inhalation/mouse Lowest published lethal concentration: 0.15 gm/m3/15MSkin: After systemic exposure: Dermatitis, otherTPKVAL 3,103,1961
inhalation/mouse lowest published toxic concentration: 0.99 ppm/30MBlood: HemorrhageTOXID9 60,426,2001
inhalation/rabbit lethal concentration (50 percent kill): 800 mg/m3/20MFMCHA2 -,C72,1991
inhalation/rat lethal concentration (50 percent kill): 14400 ppb/4HLung, Thorax, or Respiration: Other changesNNGADV 16,63,1991
inhalation/rat lethal concentration (50 percent kill): 18.9 ppm/4HOlfaction: Change in sensation of smell

Behavioral: Somnolence (general depressed activity)

Lung, Thorax, or Respiration: Other changes
TOXID9 60,426,2001
inhalation/rat lowest published toxic concentration: 10.5 ppm/4HGastrointestinal: Nausea or vomitingTOXID9 60,426,2001
inhalation/rat lowest published toxic concentration: 10.5 ppm/4HLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Lipids including transport
TOXID9 60,426,2001
inhalation/rat Lowest published lethal concentration: 18 ppm/4HOlfaction: Other olfaction effects

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Cytochrome oxidases (indlucing oxidative phosphrylation)
TOXID9 60,426,2001
inhalation/rat Lowest published lethal concentration: 23.5 ppm/4HOlfaction: Change in sensation of smell

Behavioral: Somnolence (general depressed activity)

Lung, Thorax, or Respiration: Other changes
TOXID9 60,426,2001
intraperitoneal/mouse lethal dose (50 percent kill): 25 mg/kgENTOX* -,571,2005
intravenous/guinea pig lethal dose (50 percent kill): 4200 µg/kgLung, Thorax, or Respiration: Acute pulmonary edemaAIPTAK 114,146,1958
oral/rat lethal dose (50 percent kill): 250 mg/kgDOWCC* -,-,1972

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/rat lowest published toxic dose: 400 mg/kg/10D- intermittentEndocrine: Changes in thymus weight

Blood: Changes in leukocyte (WBC) count

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
DCTODJ 17,125,1994
oral/rat lowest published toxic dose: 2880 mg/kg/90D- intermittentGastrointestinal: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
DCTODJ 17,125,1994

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 0.1 ppmDTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Not classifiable as a human carcinogenDTLVS* TLV/BEI,2013
TOXICOLOGY REVIEWMUREAV 567,347,2004
TOXICOLOGY REVIEWENTOX* -,571,2005
TOXICOLOGY REVIEWMUREAV 636,242,2007
TOXICOLOGY REVIEWMUREAV 831,1,2018
TOXICOLOGY REVIEWRTOPDW 54,247,2009

Standards and Regulations

OrganizationStandardReference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATIONFEREAC 54,7740,1989
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDESSupportedRBREV* -,98,1998
Mine Safety and Health Administration (MSHA) STANDARD-airtime-weighted average 0.1 ppm (0.7 mg/m3)DTLVS* 3,54,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 0.1 ppm (0.67 mg/m3), JUL2008
Occupational Exposure Limit-AUSTRIAMAK-TMW 0.1 ppm (0.7 mg/m3);KZW 0.2 ppm (1.4 mg/m3), 2007
Occupational Exposure Limit-BELGIUMtime-weighted average 0.1 ppm (0.68 mg/m3), MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 0.1 ppm (0.7 mg/m3), MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 0.1 ppm (0.7 mg/m3), short term exposure limit 0.3 ppm (2.1 mg/m3), NOV2011
Occupational Exposure Limit-FRANCEVME 0.1 ppm (0.7 mg/m3), FEB2006
Occupational Exposure Limit-GERMANYMAK 0.1 ppm (0.68 mg/m3), 2011
Occupational Exposure Limit-ICELANDtime-weighted average 0.1 ppm (0.7 mg/m3), NOV2011
Occupational Exposure Limit-JAPANOccupational Exposure Limit 0.1 ppm (0.67 mg/m3), MAY2012
Occupational Exposure Limit-KOREAtime-weighted average 0.1 ppm (0.7 mg/m3), short term exposure limit 0.3 ppm (2 mg/m3), 2006
Occupational Exposure Limit-MEXICOtime-weighted average 0.1 ppm (0.7 mg/m3), 2004
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 0.1 ppm (0.67 mg/m3), JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 0.1 ppm (0.7 mg/m3), JAN1999
Occupational Exposure Limit-PERUtime-weighted average 0,1 ppm (0,7 mg/m3), JUL2005
Occupational Exposure Limit-POLANDMAC(time-weighted average) 0.5 mg/m3, MAC(short term exposure limit) 1.5 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAshort term exposure limit 0.5 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWITZERLANDceiling concentration 0.1 ppm (0.7 mg/m3), JAN2011
Occupational Exposure Limit-THE NETHERLANDSMAC-TGG 0.7 mg/m3, 2003
Occupational Exposure Limit-THE PHILIPPINEStime-weighted average 0.1 ppm (0.7 mg/m3), JAN1993
Occupational Exposure Limit-TURKEYtime-weighted average 0.1 ppm (0.7 mg/m3), JAN1993
Occupational Exposure Limit-UNITED KINGDOMtime-weighted average 0.1 ppm (0.68 mg/m3);short term exposure limit 0.3 ppm (2.1 mg/m3), OCT2007
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 0.1 ppm (0.7 mg/m3)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 0.1 ppm (0.7 mg/m3)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 0.1 ppm (0.7 mg/m3)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 0.1 ppm (0.7 mg/m3)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO CHLOROPICRIN-air10H time-weighted average 0.1 ppmNIOSH* DHHS #95-121,1995
National Occupational Exposure Survey 1983Hazard Code M0419; Number of Industries 3; Total Number of Facilities 484; Number of Occupations 8; Total Number of Employees Exposed 4843; Total Number of Female Employees Exposed 939

Status in Federal Agencies

OrganizationReference
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (gavage);inadequate studies:ratNCITR* NCI-TR-65,1978
NCI Carcinogenesis Bioassay (gavage);no evidence:mouseNCITR* NCI-TR-65,1978
Page last reviewed: November 16, 2018