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Download ELISA Data Processing and Analysis Software

Note: Although this software and accompanying documentation is dated 2004-2005, it is still valid in 2014. Questions can be sent to CDC-INFO.

User's Manual

The User's Manual is the operational manual for the software.

ELISA Version 2.15

ELISA Version 2.15 [2 MB] (09/24/2004)

Instructions for Download/Installation

Microsoft Explorer download directions:

  • To save a copy of the user’s manual and the reference, right click on the underlined file name and left click on the 'Save Target As...' selection on the pull-down menu. Use the file-save dialog box to direct the file to the desired disk drive and subdirectory. Please note the reference document is 11 megabytes.
  • To save a copy of the zip files, left click on the underlined filenames and selecting 'Save' which opens the file-save dialog box. Alternatively, you may right click on the underlined file names and save the files as described, above.
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Validation Documents

This collection of reports, protocols and files serves as a validation package for the CDC Program ELISA. Each document with its associated data files is located in zip files accessed through the following links:

Introduction

The Introduction contains the following information. It also contains an complete list of data files associated with the different documents described below.

Introduction [8 pages]

A Process Verification and Qualification Development Report

Details the theoretical foundations for the method programmed into the software. This document provides mathematical formulas used to implement the methods and also details how various quantities are calculated in the program. While not a formal validation document, it does provide example calculations which are compared with published results to illustrate the calculation formulas have been implemented correctly. This document refers to sample data files which are included in the zip file. Both Adobe Acrobat (PDF) and Microsoft Word versions of the report are included. The Microsoft Word version is provided in the event a user wishes to adapt the document or use portions of it for customized applications in their research environments.

Process [829 KB]

An Installation Qualification Protocol

Provides precise instructions for the installation of the ELISA program. This protocol is provided in both Adobe Acrobat (PDF) [2 pages] and Microsoft Word [2 pages] versions. The Microsoft Word version may be used to create a specific installation protocol for use in any given research environment.

Instructions for installation

An Operational Qualification Protocol

Provides a detailed series of steps to confirm the software is installed correctly and that the program is delivering results within acceptable specifications. This protocol is provided in both Adobe Acrobat and Microsoft Word versions. The Microsoft Word version may be used to create a specific operational protocol for use in any given research environment. The associated files that accompany this protocol are included in the zip file.

Operational Qualification Protocol [1 MB]

A Validation Protocol and Report

Describes the formal validation procedures for the software and reports the results of the validation process. These are the documents that describe the formal validation of the ELISA program. Both Adobe Acrobat and Microsoft Word versions of the protocol and report are included. The Microsoft Word version is provided in the event a user wishes to adapt the documents or use portions of them for customized applications in their research environments. The associated data files that accompany the report are included in the zip file.

Validation Protocol and Report [667 KB]

Reference

Contains the original reference that lays the foundation for the use of the robust fitting techniques in the four-parameter logistic-log function: B. D. Plikaytis, G. M. Carlone, P. Edmonds, and L. W. Mayer, Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base-pair number after gel electrophoresis, (1986) Analytical Biochemistry, 152:346-364. Erratum: 154:702 (1986).

Reference [11 MB, 20 pages]

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