Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
Help Glossary Quit    
1 of 11
Menu

Next Step

Site Contents

Home

About this course
Course Topics

Disease Vectors

Strategies for Resistance Management

Resistance Assays
Bottle Bioassay
Larval Bioassay
Microplate Assay
Interpretation of Results

Case Studies
Supplements

Links and Resources

Companion Materials

Send a question or comment
Microplate Assays

Materials Needed (where to get materials)

  • 1.5 ml tubes
  • pestles for above tubes
  • pipettors and tips
  • multichannel pipetors
  • forceps or tweezers
  • freezer (used to kill/stun samples)
  • timer (digital counter capable of counting seconds)
  • microplates
  • microplate reading spectrophotometer
  • analitical balance
  • bottles of various sizes to store mixed chemicals
  • graduated cylinders
  • pH meter

Overview

This procedure has eight steps. Steps are repeated for each insecticide formulation used.

Step 1:

Preserving samples (handling of mosquitoes)
Step 2:

Preparing stock solutions
Step 3:

Preparing control solutions
Step 4:

Preparing homogenates
Step 5: Loading homogenates into microplates
Step 6: Adding reagents
Step 7: Collecting data
Step 8: Interpreting data and using the diagnostic dosage to detect resistance
Step 9: Clean up
  Practice questions

Next Step
Go to Top

| Help | Glossary | Quit | Previous | Menu | Next

| NCID Home | | CDC Home | Search | Health Topics A-Z |

This page last reviewed May 7, 2002

Centers for Disease Control and Prevention
National Center for Infectious Diseases

Privacy Policy | Accessibility