Larval Bioassays
The larval
bioassay allows resistance levels to be established for populations of
larvae reared in breading pan or collected in the field. The major advantages
of the bottle assay are that any concentration of any insecticide may
be evaluated at the one time. Secondly, the technique is simple and rapid.
The goal
of the bioassay is to measure the time it takes for a given insecticide
to kill the larvae.
Materials
Needed (where to get materials)
- 150 ml
glass beakers
- wide-mouth
pipettors (used to transfer larve to beakers)
- pipetor
and tips (one for each insecticide sample)
- absolute
alcohol
- chemical(s)
to be tested
- stirring
rod or similar equipment
- disposable
plastic or wooden probes (toothpicks)
- timer
(digital counter capable of counting seconds)
- forceps
or tweezers
- late
3rd or early 4th instar mosquito larvae
Overview
This procedure
takes four steps. Steps are repeated for each insecticide formulation
used.
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