bioassay allows resistance levels to be established for populations of
larvae reared in breading pan or collected in the field. The major advantages
of the bottle assay are that any concentration of any insecticide may
be evaluated at the one time. Secondly, the technique is simple and rapid.
of the bioassay is to measure the time it takes for a given insecticide
to kill the larvae.
Needed (where to get materials)
- 150 ml
pipettors (used to transfer larve to beakers)
and tips (one for each insecticide sample)
to be tested
rod or similar equipment
plastic or wooden probes (toothpicks)
(digital counter capable of counting seconds)
3rd or early 4th instar mosquito larvae
takes four steps. Steps are repeated for each insecticide formulation