Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 Resistance Assays
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 Larval Bioassay
 Microplate Assay
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Bottle Bioassay > Timing and plotting mosquito mortality

Scoring data

Data are recorded at 10 minute intervals. The first count should be made as soon as the mosquitoes are in the bottle.

Run four replicates of each insecticide plus one control (view sample run).

Mosquitoes are scored as alive or dead. It helps to gently rotate the bottle while counting.

Dead mosquitoes that slide with the curvature of the bottle are more easily scored.

It is easier to count the number of dead mosquitoes early in the test and easier to count live mosquitoes when few live ones remain.

 
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This page last reviewed May 7, 2002

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