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Volume 8, Number 1, January 2002

Research

A Large Outbreak of Legionnaires' Disease at a Flower Show, the Netherlands, 1999

Jeroen W. Den Boer,*† Ed P.F. Yzerman,‡ Joop Schellekens,* Kamilla D. Lettinga,§ Hendriek C. Boshuizen,* Jim E. Van Steenbergen,¶ Arnold Bosman,* Susan Van den Hof,* Hans A. Van Vliet,* Marcel F. Peeters,# Ruud J. Van Ketel,§ Peter Speelman,§ Jacob L. Kool,* and Marina A.E. Conyn-Van Spaendonck*
*National Institute of Public Health and the Environment, Bilthoven, the Netherlands; †Municipal Health Service Zuid-Kennemerland, Haarlem, the Netherlands; ‡Regional Laboratory of Public Health Haarlem, Haarlem, the Netherlands; §Academic Medical Center, Amsterdam, the Netherlands; ¶National Outbreak Structure for Infectious Diseases, Den Haag, the Netherlands; and #Regional Laboratory of Public Health Tilburg, Tilburg, the Netherlands

Figure 5. Pulsed-field gel electrophoresis (PFGE) and amplified fragment-length polymorphism (AFLP) patterns of a representative selection of clinical and environmental Legionella pneumophila isolates; the dendrogram shows clustering in PFGE. The AFLP and PFGE pattern of the isolate of patient 15 (genotype B-1) was found in 28 of the 29 isolates of culture-positive cases; the same pattern was found in isolates cultured from the whirlpool spas in halls 3 and 4. The AFLP and PFGE pattern of the isolate of patient 25 (genotype B-2) was unique among culture-positive cases; the same pattern was found in isolates cultured from the whirlpool spa in hall 3 and the sprinkler.

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Figure 5. Pulsed-field gel electrophoresis (PFGE) and amplified fragment-length polymorphism (AFLP) patterns of a representative selection of clinical and environmental Legionella pneumophila isolates; the dendrogram shows clustering in PFGE. The AFLP and PFGE pattern of the isolate of patient 15 (genotype B-1) was found in 28 of the 29 isolates of culture-positive cases; the same pattern was found in isolates cultured from the whirlpool spas in halls 3 and 4. The AFLP and PFGE pattern of the isolate of patient 25 (genotype B-2) was unique among culture-positive cases; the same pattern was found in isolates cultured from the whirlpool spa in hall 3 and the sprinkler.
 


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