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Volume 8, Number 1, January 2002

Dispatch

Absence of Mycoplasma Contamination in the Anthrax Vaccine

Mary Kate Hart,* Richard A. Del Giudice,† and George W. Korch, Jr.*
*United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA; and †National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland, USA

Figure. Evaluation of Anthrax Vaccine Adsorbed (AVA) for amplified mycoplasma DNA by gel electrophoresis. Molecular weight markers were run in lanes 1 and 17. Control samples in lanes 2-5 were mycoplasma broth, Hut 78 cell extract, Acholeplasma laidlawii, and Mycoplasma pirum, respectively. The AVA samples were in lanes 6 to 15: Lot FAV048B from Davis Monthan AFB (lane 6), Kansas City MO NRC (lane 7), and Camp Pendleton (lane 8); Lot FAV047 from Fort Detrick (lanes 9 and 11) and Pearl Harbor NMC (lane 10); Lot FAV031 from Fort Worth Base Naval Clinic (lane 12) and the Pentagon Clinic (lane 13); and Lot FAV008 from Davis Monthan AFB (lane 14) and McEntire ANG Station (lane 15). Lane 16 contained water. Bands seen below 100 base pairs are primer multimers.

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Figure. Evaluation of Anthrax Vaccine Adsorbed (AVA) for amplified mycoplasma DNA by gel electrophoresis. Molecular weight markers were run in lanes 1 and 17. Control samples in lanes 2-5 were mycoplasma broth, Hut 78 cell extract, Acholeplasma laidlawii, and Mycoplasma pirum, respectively. The AVA samples were in lanes 6 to 15: Lot FAV048B from Davis Monthan AFB (lane 6), Kansas City MO NRC (lane 7), and Camp Pendleton (lane 8); Lot FAV047 from Fort Detrick (lanes 9 and 11) and Pearl Harbor NMC (lane 10); Lot FAV031 from Fort Worth Base Naval Clinic (lane 12) and the Pentagon Clinic (lane 13); and Lot FAV008 from Davis Monthan AFB (lane 14) and McEntire ANG Station (lane 15). Lane 16 contained water. Bands seen below 100 base pairs are primer multimers.
 


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