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Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen,*† Matti K. Viljanen,* Jussi Mertsola,† Heikki Arvilommi,* and Qiushui He*
*National Public Health Institute, Department in Turku, Finland; †Turku University Central Hospital, Turku, Finland

Figure 5A. Melting curves from the allele-specific amplification assay, showing the presence of amplified products from prn1-5 and the absence of amplification from prn6-8 and the negative control. B. Corresponding melting peaks derived from the melting curve. Prn1 represents the prn1-5 types; prn7 represents prn6-8 types and the negative control.

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Figure 5. A. Melting curves from the allele-specific amplification assay, showing the presence of amplified products from prn1-5 and the absence of amplification from prn6-8 and the negative control. B. Corresponding melting peaks derived from the melting curve. Prn1 represents the prn1-5 types; prn7 represents prn6-8 types and the negative control.
 


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This page last reviewed December 08, 2001

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National Center for Infectious Diseases
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