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Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen,*† Matti K. Viljanen,* Jussi Mertsola,† Heikki Arvilommi,* and Qiushui He*
*National Public Health Institute, Department in Turku, Finland; †Turku University Central Hospital, Turku, Finland

Figure 3. Schematic structure of the prn1 gene, showing the position of the primers used in the allele-specific amplification assay (1.), and the primers and probes used in the fluorescence resonance energy transfer probe assay (2.). Proportions of the gene are not drawn to scale. F = fluorescein; LC = LC red 640; and P = phosphate.

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Figure 3. Schematic structure of the prn1 gene, showing the position of the primers used in the allele-specific amplification assay (1.), and the primers and probes used in the fluorescence resonance energy transfer probe assay (2.). Proportions of the gene are not drawn to scale. F = fluorescein; LC = LC red 640; and P = phosphate.
 


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This page last reviewed December 08, 2001

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National Center for Infectious Diseases
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