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Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen,*† Matti K. Viljanen,* Jussi Mertsola,† Heikki Arvilommi,* and Qiushui He*
*National Public Health Institute, Department in Turku, Finland; †Turku University Central Hospital, Turku, Finland

Figure 1. Workflow for typing prn alleles. The allele-specific amplification (ASA) assay (step number 3) and the fluorescence resonance energy transfer (FRET) probe assay (step number 4) each require approximately 1 hour.

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Figure 1. Workflow for typing prn alleles. The allele-specific amplification (ASA) assay (step number 3) and the fluorescence resonance energy transfer (FRET) probe assay (step number 4) each require approximately 1 hour.
 


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This page last reviewed December 08, 2001

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention