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 Research

Molecular Identification of Streptomycin Monoresistant Mycobacterium tuberculosis Related to Multidrug-Resistant W Strain

Pablo Bifani,*† Barun Mathema,* Martha Campo,* Soraya Moghazeh,* Beth Nivin,‡ Elena Shashkina,* Jeffrey Driscoll,§ Sonal S. Munsiff,‡ Richard Frothingham,¶ and Barry N. Kreiswirth*
*Public Health Research Institute Tuberculosis Center, New York, NY, USA; †New York University School of Medicine, New York, New York, USA; ‡New York City Department of Health, New York, New York, USA; §New York State Department of Health, Albany, New York, USA; ¶Durham Veterans Affairs Medical Center, Durham, North Carolina


Figure 3. Southern blot hybridization with the DRab probe. The DRab probe was used to hybridize against the PvuII-digested chromosomal DNA previously used for IS6110 fingerprinting. Members of the W14 subgroup had a single hybridizing band of a slight lower molecular weight than that of other W variants. In contrast, strain CDC1551, which is known to have an insertion within the direct-repeat locus, yielded two hybridizing bands, including one of higher molecular weight. Both hybridizing bands in strain CDC1551 can be superimposed with the IS6110 patterns of this isolate.

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Figure 3. Southern blot hybridization with the DRab probe. The DRab probe was used to hybridize against the PvuII-digested chromosomal DNA previously used for IS6110 fingerprinting. Members of the W14 group had a single hybridizing band of a slight lower molecular weight than that of other W variants. In contrast, strain CDC1551, which is known to have an insertion within the direct-repeat locus, yielded two hybridizing bands, including one of higher molecular weight. Both hybridizing bands in strain CDC1551 can be superimposed with the IS6110 patterns of this isolate.
 


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