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 Research

Molecular Identification of Streptomycin Monoresistant Mycobacterium tuberculosis Related to Multidrug-Resistant W Strain

Pablo Bifani,*† Barun Mathema,* Martha Campo,* Soraya Moghazeh,* Beth Nivin,‡ Elena Shashkina,* Jeffrey Driscoll,§ Sonal S. Munsiff,‡ Richard Frothingham,¶ and Barry N. Kreiswirth*
*Public Health Research Institute Tuberculosis Center, New York, NY, USA; †New York University School of Medicine, New York, New York, USA; ‡New York City Department of Health, New York, New York, USA; §New York State Department of Health, Albany, New York, USA; ¶Durham Veterans Affairs Medical Center, Durham, North Carolina

A
Figure 1. Southern blot hybridization of Mycobacterium tuberculosis isolates. A) IS6110-3' was used as a hybridization probe. The bracketed pattern motives regions are characteristic of the W14 family. A1 and NTF denote the bands corresponding to the IS6110 insertions in the dnaA-dnaN region and the NTF locus, respectively. Lanes 1 and 9 are standard markers; lane 2: W-MDR from New York City (W index strain); lane 3, 4, 5 and 7: members of the W14 family; lane 6: W76 and lane 8: laboratory control strain H37Ra. B) Southern blot hybridization with IS6110-5' probe. W23 and W26 each have one additional band from W14, when hybridized with either the 3' or 5' IS6110 probe. C) The polymorphic GC-rich repetitive sequence was used as a probe. The W14 group (W14; W23; W26) has a distinctive pattern when compared to all other isolates typed by polymorphic GC-rich repetitive sequence probe.
B
Figure 1. Southern blot hybridization of Mycobacterium tuberculosis isolates. A) IS6110-3' was used as a hybridization probe. The bracketed pattern motives regions are characteristic of the W14 family. A1 and NTF denote the bands corresponding to the IS6110 insertions in the dnaA-dnaN region and the NTF locus, respectively. Lanes 1 and 9 are standard markers; lane 2: W-MDR from New York City (W index strain); lane 3, 4, 5 and 7: members of the W14 family; lane 6: W76 and lane 8: laboratory control strain H37Ra. B) Southern blot hybridization with IS6110-5' probe. W23 and W26 each have one additional band from W14, when hybridized with either the 3' or 5' IS6110 probe. C) The polymorphic GC-rich repetitive sequence was used as a probe. The W14 group (W14; W23; W26) has a distinctive pattern when compared to all other isolates typed by polymorphic GC-rich repetitive sequence probe.
C
Figure 1. Southern blot hybridization of Mycobacterium tuberculosis isolates. A) IS6110-3' was used as a hybridization probe. The bracketed pattern motives regions are characteristic of the W14 family. A1 and NTF denote the bands corresponding to the IS6110 insertions in the dnaA-dnaN region and the NTF locus, respectively. Lanes 1 and 9 are standard markers; lane 2: W-MDR from New York City (W index strain); lane 3, 4, 5 and 7: members of the W14 family; lane 6: W76 and lane 8: laboratory control strain H37Ra. B) Southern blot hybridization with IS6110-5' probe. W23 and W26 each have one additional band from W14, when hybridized with either the 3' or 5' IS6110 probe. C) The polymorphic GC-rich repetitive sequence was used as a probe. The W14 group (W14; W23; W26) has a distinctive pattern when compared to all other isolates typed by polymorphic GC-rich repetitive sequence probe.

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Figure 1. Southern blot hybridization of Mycobacterium tuberculosis isolates. A) IS6110-3' was used as a hybridization probe. The bracketed pattern motives regions are characteristic of the W14 family. A1 and NTF denote the bands corresponding to the IS6110 insertions in the dnaA-dnaN region and the NTF locus, respectively. Lanes 1 and 9 are standard markers; lane 2: W-MDR from New York City (W index strain); lane 3, 4, 5 and 7: members of the W14 family; lane 6: W76 and lane 8: laboratory control strain H37Ra. B) Southern blot hybridization with IS6110-5' probe. W23 and W26 each have one additional band from W14, when hybridized with either the 3' or 5' IS6110 probe. C) The polymorphic GC-rich repetitive sequence was used as a probe. The W14 group (W14; W23; W26) has a distinctive pattern when compared to all other isolates typed by polymorphic GC-rich repetitive sequence probe.
 


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This page last reviewed December 08, 2001

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
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