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West Nile Virus

Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay

Donna J. Johnson, Eileen N. Ostlund, Douglas D. Pedersen, and Beverly J. Schmitt
Animal and Plant Health Inspection Service, U. S. Department of Agriculture, Ames, Iowa, USA

Figure 2. Visual comparison of first stage reverse transcription-polymerase chain reaction (RT-PCR) amplification products with RT-nested (n) PCR amplification products. Lanes 1-9 represent first-stage products of 10-fold dilutions 10-1 through 10-9. Lanes 10-18 represent nested amplification products of same dilutions. L: 100-bp DNA ladder.

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Figure 2. Visual comparison of first stage reverse transcription-polymerase chain reaction (RT-PCR) amplification products with RT-nested (n) PCR amplification products. Lanes 1-9 represent first-stage products of 10-fold dilutions 10-1 through 10-9. Lanes 10-18 represent nested amplification products of same dilutions. L: 100-bp DNA ladder.
 


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This page last reviewed December 08, 2001

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention