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West Nile Virus

Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay

Donna J. Johnson, Eileen N. Ostlund, Douglas D. Pedersen, and Beverly J. Schmitt
Animal and Plant Health Inspection Service, U. S. Department of Agriculture, Ames, Iowa, USA


Figure 1. Visualization of reverse transcription-nested polymerase chain reaction product. West Nile virus-positive samples are indicated by a 248-bp band. Lane 1: positive crow brain, NY, 1999. Lane 2: positive crow kidney, NY, 1999. Lane 3: positive Sandhill Crane brain, CT, 1999. Lane 4: negative crow brain. Lane 5-6: positive crow brains, NY, 2000. Lane 7: normal control. Lane 8-10: positive horse brains, NY, 1999. Lane 11: negative horse brain. Lane 12: positive horse brain, RI, 2000. Lane 13: positive horse brain, NJ, 2000. Lane 14: positive horse brain, MA, 2000. Lane 15: positive horse brain, CT, 2000. Lane 16: normal control. Lane 17: NY99 West Nile virus control. L: 100-bp DNA ladder. Band artifacts in lanes 1, 3, 5, and 6 are due to large amount of virus present in those samples.

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Figure 1. Visualization of reverse transcription-nested polymerase chain reaction product. West Nile virus-positive samples are indicated by a 248-bp band. Lane 1: positive crow brain, NY, 1999. Lane 2: positive crow kidney, NY, 1999. Lane 3: positive Sandhill Crane brain, CT, 1999. Lane 4: negative crow brain. Lane 5-6: positive crow brains, NY, 2000. Lane 7: normal control. Lane 8-10: positive horse brains, NY, 1999. Lane 11: negative horse brain. Lane 12: positive horse brain, RI, 2000. Lane 13: positive horse brain, NJ, 2000. Lane 14: positive horse brain, MA, 2000. Lane 15: positive horse brain, CT, 2000. Lane 16: normal control. Lane 17: NY99 West Nile virus control. L: 100-bp DNA ladder. Band artifacts in lanes 1, 3, 5, and 6 are due to large amount of virus present in those samples.
 


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This page last reviewed December 08, 2001

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention