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Research Waterborne Outbreak of Tularemia Associated with Crayfish FishingPedro Anda,* Javier Segura del Pozo,*† José María Díaz García,‡ Raquel
Escudero,* F. Javier García Peña,§ M. Carmen López Velasco,‡ Ricela E.
Sellek,* M. Rosario Jiménez Chillarón,‡ Luisa P. Sanchez Serrano,* and J. Fernando Martínez Navarro* |
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| Back to article Figure 4. Polymerase chain reaction results in selected samples from water, crayfish, patients, and controls. Lanes 1 and 2: positive control (first [1550-bp] and second [950-bp] rounds of amplification); lanes 3 and 4: water samples 1 and 3, respectively, from the sewage plant, second round; lanes 5 and 6: stomach and hepatopancreas, respectively, from batch A of crayfish, second round; lane 7: negative control; lanes 8 and 10: control of amplification of 1 CFU for the first and second rounds of amplification, respectively; lanes 11 and 12: human lymph node aspirate, first and second amplifications, respectively; lanes 13 and 14: positive control, first round; lanes 15-19,21, and 22: water samples from the river; lanes 23 and 24: stomach and hepatopancreas, respectively, from batch B of crayfish; lanes 25 and 26: pooled DNA from Salmonella Typhimurium, Escherichia coli, Legionella pneumophila, Yersinia enterocolitica, and Proteus vulgaris (first and second rounds of amplification, respectively); lanes 9 and 20, 1-kb DNA ladder size standards. |
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CDC Home | Search | Health Topics A-Z This page last reviewed June 14, 2001 Emerging Infectious Diseases Journal
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![Figure 4. Polymerase chain reaction results in selected samples from water, crayfish, patients, and controls. Lanes 1 and 2: positive control (first [1550-bp] and second [950-bp] rounds of amplification); lanes 3 and 4: water samples 1 and 2, respectively, from the sewage plant, second round; lanes 5 and 6: stomach and hepatopancreas, respectively, from batch A of crayfish, second round; lane 7: negative control; lanes 8 and 10: control of amplification of 1 CFU for the first and second rounds of amplification, respectively; lanes 11 and 12: human lymph node aspirate, first and second amplifications, respectively; lanes 13 and 14: positive control, first round; lanes 15-19,21, and 22: water samples from the river; lanes 23 and 24: stomach and hepatopancreas, respectively, from batch B of crayfish; lanes 25 and 26: pooled DNA from Salmonella Typhimurium, Escherichia coli, Legionella pneumophila, Yersinia enterocolitica, and Proteus vulgaris (first and second rounds of amplification, respectively); lanes 9 and 20, 1-kb DNA ladder size standards.](images/anda4b.jpg)