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Research

Outbreak of Human Monkeypox, Democratic Republic of Congo, 1996 to 1997

Yvan J.F. Hutin,* R. Joel Williams,* Philippe Malfait,† Richard Pebody,† Vladamir N. Loparev,* Susan L. Ropp,* Mariangelli Rodriguez,* Janice C Knight,* Florimont K. Tshioko,‡ Ali S Khan,* Mark V. Szczeniowski,‡ and Joseph J. Esposito*
*Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †European Programme for Intervention Epidemiology Training, Brussels, Belgium; and ‡World Health Organization, Geneva, Switzerland


Figure 2. Phylogenetic inference relationships of the open reading frames encoding the viral hemagglutinin protein of various monkeypox virus isolates and selected strains of vaccinia, variola, and cowpox viruses. Nucleotide sequences of polymerase chain reaction-generated amplicons were analyzed using PAUP parsimony analysis software version 3.1.1, as described (10). Parsimony analysis used 5,000 bootstraps and weighted the sequences for a transition-transversion ratio of 2 (bootstrap confidence intervals shown on branches).

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Figure 2. Phylogenetic inference relationships of the open reading frames encoding the viral hemagglutinin protein of various monkeypox virus isolates and selected strains of vaccinia, variola, and cowpox viruses. Nucleotide sequences of polymerase chain reaction-generated amplicons were analyzed using PAUP parsimony analysis software version 3.1.1, as described (10). Parsimony analysis used 5,000 bootstraps and weighted the sequences for a transition-transversion ratio of 2 (bootstrap confidence intervals shown on branches).
 


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This page last reviewed May 19, 2001

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention