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Research

Emerging Chagas Disease: Trophic Network and Cycle of Transmission of Trypanosoma cruzi from Palm Trees in the Amazon

Antonio R.L. Teixeira,* Pedro Sadi Monteiro,* José M. Rebelo,† Enrique R. Argañaraz,* Daniela Vieira,* Liana Lauria-Pires,* Rubens Nascimento,* Cássia A. Vexenat,* Antonio R. Silva,† Steven K. Ault,‡ and Jackson M. Costa‡
*University of Brasília, †Federal University of Maranhão, and ‡Pan-American Health Organization/World Health Organization, Brasília, Brazil

Figure 7. In situ hybridization of Rhodnius pictipes-derived flagellates with an archetypic Berenice Trypanosoma cruzi-specific probe. The byotinylated 198-bp sequence amplified with Tcz1/2 nuclear DNA probe (37) was revealed with fluorescein-conjugated streptavidin. This genotypic marker, representing 12.5% of total parasitic nuclear DNA, also confirms this sylvatic isolate as T. cruzi.

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Figure 7. In situ hybridization of Rhodnius pictipes-derived flagellates with an archetypic Berenice Trypanosoma cruzi-specific probe. The byotinylated 198-bp sequence amplified with Tcz1/2 nuclear DNA probe (37) was revealed with fluorescein-conjugated streptavidin. This genotypic marker, representing 12.5% of total parasitic nuclear DNA, also confirms this sylvatic isolate as T. cruzi.
  

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Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention