Imported Lassa Fever in Germany: Molecular Characterization of a New Lassa Virus Strain

	*Research* Imported Lassa Fever in Germany: Molecular Characterization of a New Lassa Virus Strain Stephan Günther, Petra Emmerich, Thomas Laue, Olaf Kühle, Marcel Asper, Annegret Jung, Thomas Grewing, Jan ter Meulen, and Herbert Schmitz Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

	Back to article Figure 3. Reverse transcription (RT) and polymerase chain reaction (PCR) amplification of full-length S RNA. (A) Position of the RT and PCR primers at the termini of S RNA. The stem-loop structure in the intergenic region is schematically shown. (B) Amplified S RNA of Lassa Josiah and LCMV Armstrong virus separated in ethidium bromide-stained agarose gel. S RNA was isolated from supernatant of infected cells, and PCR was done with primers PCR2-4. (C) S RNA of Lassa AV was amplified in two RT-PCRs (a and b) and separated in ethidium bromide-stained agarose gel. RNA was isolated from serum, and PCR was performed with primers PCR2-4. Quantification of Lassa virus RNA in the specimen by endpoint titration with the 340-bp PCR assay showed >10⁶ S RNA molecules/mL serum.

	Home \| *Top of Page \| Current Issue \| Expedited* \| Upcoming Issue \| Past Issue \| EID Search \| Contact Us CDC Home \| Search \| Health Topics A-Z** This page last reviewed August 1, 2000 Emerging Infectious Diseases Journal National Center for Infectious Diseases Centers for Disease Control and Prevention