Figure 1. Pulsed-field gel electrophoresis of SfiI restriction fragments of Streptococcus pyogenes DNAs of erythromycin-resistant S. pyogenes. Lanes 1-2, MLS_b phenotype strains; lane 3, clone F (biotype I, type T28, emm28); lanes 4-5, clone A (biotype III, type T12, emm12); lanes 6-7, clone B (biotype I, type T4, emm4); lanes 8-9, clone C (biotype I, type T4, emm4); lane 10, clone D (biotype V, type T8.25, emm75); lanes 11-12, clone E (biotype I, type T1, emm1); lane 13, DNA size standards (lambda ladder, 48.5 to 1,018 kb); lanes 14 and 16, clone H (biotype III, type TB3264, emm2); and lane 15, clone G (biotype I, type TB3264 emm not typeable).

Figure 2. A dendrogram showing the genetic relationship of 15 clones of M-phenotype erythromycin-resistant Streptococcus pyogenes and two erythromycin-sensitive strains (ErS) established from pulsed-field gel electrophoresis patterns obtained after SfiI digestion by using the Dice coefficient and UPGMA and Lane Manager 2.1 software. Clone A (III/T12/emm12^a); Clone B (I/T4/emm4); Clone C (I/T4/emm4); Clone D (V/T8,25/emm75); Clone E (I/T1/emm1); Clone F (I/T28/emm28); Clone G (I/ TB3264/emm nt^b); Clone H (III/TB3264/emm2); Clone I (I/T4/emm4); Clone J (V/T8,25/emm75); Clone K (II/T2/emm2); Clone L (III/T nt/ emm nt); Clone M (III/T12/emm12); Clone N (III/T12/emm12); Clone O (III/Tnt/emm nt); ErS T12 (III/T12/emm12); ErS T4 (I/T4/emm4).
^aBiotype/T-serotype/emm type.
^bnt = nontypeable.