Volume 12, Number 1, January 2006

SARS–associated Coronavirus Replication in Cell Lines

Matthew Kaye,* Julian Druce,* Thomas Tran,* Renata Kostecki,* Doris Chibo,* Jessica Morris,* Mike Catton,* and Chris Birch*
*Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia

Figure 2. Cycle threshold (Ct) changes measured by real-time polymerase chain reaction versus days after infection of the indicated cell lines. The cell-free sample had an initial Ct of 31, which rose to 40 by day 11. Reductions in the Ct or flat-line Ct values (e.g., COS cells) indicate replication of the virus. Continued increases in Ct above the initial value of 31 by days 7 and 11 indicate failure to replicate.

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Emerging Infectious Diseases Journal
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