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Figure 2. Phylogenetic tree resulting from analysis of selected
351-bp fragments of herpesvirus DNA polymerase gene, which is available
for all viruses. The DNA sequences were first aligned by using ClustalX
(nonphylogenetic informative gaps were manually removed), then the phylogeny
was derived by the neighbor-joining method applied to pairwise sequence
distances calculated by the Kimura two-parameter method (transition-to-transversion
ratio set at 1.15 as expected by a previous Maximum Likelihood analysis).
Horizontal branch lengths are drawn to scale, with the bar indicating
0.1 nucleotide replacements per site. Numbers at each node indicate the
percentage of bootstrap samples (of 1,000) in which the cluster to the
right is supported. Brackets on the right indicate previously defined
subfamily and genus herpesviral classification. Previously published sequences
and their accession numbers are the following: bovine herpesvirus 4 (BHV4)
(AF031811), murine herpesvirus 68 (MHV68) (U97553), alcelaphine herpesvirus
1 (AHV1) (AF005370), ovine herpesvirus 2 (OHV2) (AF031812), bovine lymphotropic
herpesvirus (BLHV) (AF031808), equine herpesvirus 2 (EHV2) (U20824), gorilla
lymphocryptovirus 1 called here GorLHV1 a (AF290600, AF250885, and AF250884),
GgorLCV1 (AF534225), and GorLHV1 b (AF250883), Pan troglodytes
lymphocryptovirus 1 (PtroLCV1) (AF534226), Pan paniscus lymphocryptovirus
1 (PpanLCV1) (AF534220), EBV (V01555), Cercocebus aterrimus lymphocryptovirus
1 (CateLCV1) (AY174067), Mandrillus sphinx lymphocryptovirus 2
(MsphLCV2) (AY174066), Mandrillus sphinx Lymphocryptovirus 1 MsphLCV1
(AF534227), Colobus guereza lymphocryptovirus 1 (CgueLCV1) (AF534219),
Papio hamadryas lymphocryptovirus 1 (PhamLCV1) (AY174069), Papio
hamadryas lymphocryptovirus 2 (PhamLCV2) (AF534229), Macaca
lymphocryptovirus strains from Macaca sylvanus (MsylLCV1) (AY172956),
Macaca fuscata (MfusLCV1) (AF534224), Macaca mulatta (MmulLCV1)
(AY172955), Macaca fascicularis (MfasLCV1) (AF534221), and Macaca
silenus (MsilLCV1) (AF534222), Piliocolobus badius lymphocryptovirus
1 (PbabLCV1) (AF534228), Cercopithicine herpesvirus 15 (cercoHV15) (AY037858),
Semnopithecus entellus lymphocryptovirus 1 (SentLCV1) (AF534223),
Pongo pygmaeus lymphocryptovirus 1 (PpygLCV1) (AY129398), Gorilla
gorilla lymphocryptovirus 2 (GgorLCV2) (AY129395), Hylobates leucogenys
lymphocryptovirus 1 (HleuLCV1) (AY174068), H. lar lymphocryptovirus
1 (HlarLCV1) (AY196147), Saguinus midas lymphocryptovirus 1 (SmiLHV1)
(AY166691, AY166692, AY166693, AY166694, and AY166695), callitrichine
herpesvirus 3 (CalHV3) (AF319782), Callithrix penicillata lymphocryptovirus
1 (CpenLCV1) (AY139026), C. jacchus lymphocryptovirus 1 (CjacLCV1)
(AY174064), Pithecia pithecia lymphocryptovirus 1 (PpiLHV1) (AY166696
and AY166698) and PpitLCV1 (AY139025), Ateles paniscus lymphocryptovirus
1 (ApanLCV1) (AY139028), Saimiri sciureus lymphocryptovirus 1 (SsciLCV1)
(AY172953), Cebus albifrons lymphocryptovirus 1 (CalbLCV1) (AY139027),
Saimiri sciureus lymphocryptovirus 2 (SsciLCV2) (AY139024) and
SscLHV1 (AY166697), Mandrillus cytomegalovirus (MndCMVa) (AF282941)
and MndCMVb (AF387665), baboon cytomegalovirus (babCMV) (AF387664), vervet
cytomegalovirus vervet (CMV) (AY049066), cercopithecine herpesvirus 3
(cercoHV3) (AY049065), rhesus cytomegalovirus (RhCMV) (AY186194), Pan
troglodytes γ-herpesvirus (AF292064), chimpanzee cytomegalovirus
(CCMV) (AF480884), human cytomegalovirus (HCMV) (M14709), Saimiri scireus
γ-herpesvirus (AF292065), Cebus γ-herpesvirus (AF292067),
mouse cytomegalovirus 1 (MCMV) (U68299), human herpesvirus-7 (HHV7) (U43400),
Mandrillus herpesvirus-β (MndHVB) (AF282942), Nycticebus
γ-herpesvirus (AF292064), herpes simplex virus type 1 (HSV1) (X04771),
herpes simplex virus type 2 (HSV2) (M16321), bovine herpesvirus type 1
(BHV1) (Z78205), equine herpesvirus 1 (EHV1) (M86664), pseudorabies virus
(PRV) (L24487), gallid herpesvirus 2 (GHV2) (L40431) and varicella-zoster
virus (VZV) (X04370).
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