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Figure 1. Neighbor-joining protein distance tree for the best
130 amino acids of DNA polymerase residues alignment. DNA sequences (primers
DFASA and GDTD1B) (8) were first translated,
then aligned by using ClustalX (nonphylogeneticaly informative gaps were
manually removed) and analyzed by using the PROTDIST and NEIGHBOR programs
in PHYLIP (available from http://evolution.genetics.washington.edu/phylip.html).
Horizontal branch lengths are drawn to scale, with the bar indicating
0.1 amino acid replacements per site. Numbers in each internal branch
indicate the percentage of bootstrap samples (of 1,000) in which the cluster
is supported (SEQBOOT). Previously published sequences included and their
accession numbers are as follows: HyloRHV2 (AY465375); PanRHV2 (AF346490);
KSHV (U75698, U93872 and AF005477); PanRHV1a (AF250879 and AF250880);
PanRHV1b (AF250881 and AF250882); GorRHV1 (AF250886); MndRHV1 (AF282943);
MndRHV2 (AF282937 to AF282940); herpesvirus saimiri (HVS) (M31122); ateline
herpesvirus 3 (HVA3) (AF083424); Chlorocebus rhadinovirus 1 (ChRV1) (AJ251573);
ChRV2 (AJ251574); retroperitoneal fibromatosis–associated herpesvirus
strains from Macaca mulatta (AF005479) and M. nemestrina
(AF005478) called here RFHVMm and RFHVMn, respectively; M. mulatta
rhadinovirus RRV/17577 (AF083501); rhesus monkey rhadinovirus (RRV/H26-95)
(AF029302); baboon γ-herpesvirus (PapRV2) (AY270026); Macaca
γ-virus strains from M. mulatta (MGVMm) (AF159033), M.
fascicularis (MGVMf) (AF159032), and M. nemestrina (AF159031);
and HHV6A (X83413). Other sequences used to construct the phylogenetic
trees (especially Epstein-Barr virus and related strains) have been published
mainly by Ehlers et al. (9) and are named
in the legend of Figure 2.
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